Protein Info for AZOBR_RS07765 in Azospirillum brasilense Sp245

Annotation: O-acetylhomoserine aminocarboxypropyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 9 to 426 (418 residues), 595 bits, E=3.5e-183
• PF01053: Cys_Met_Meta_PP
  • amino acids 10 to 426 (417 residues), 413.8 bits, E=1e-127
• PF01041: DegT_DnrJ_EryC1
  • amino acids 63 to 188 (126 residues), 49.2 bits, E=9.4e-17
• PF00155: Aminotran_1_2
  • amino acids 69 to 187 (119 residues), 25.9 bits, E=1.1e-09

Best Hits

Swiss-Prot: 52% identical to METY_LEPME: O-acetyl-L-homoserine sulfhydrylase (metY) from Leptospira meyeri

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 89% identity to azl:AZL_018560)

MetaCyc: 52% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (3/5 steps found)
• homocysteine and cysteine interconversion (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See G8AL75 at UniProt or InterPro

Protein Sequence (429 amino acids)

>AZOBR_RS07765 O-acetylhomoserine aminocarboxypropyltransferase (Azospirillum brasilense Sp245)
MSEQKSFGFETRAIHAGAAPDPATGARQTPIYQTTSFVFDDVDDAASLFNLQKVGFIYSR
LTNPTVSVLEERLANLEGGAGATATSSGHAAQLLALFPLMEPGDEIVASRKLYGGTLNQL
GTSFPRAFGWKSVFVDTDQPENVRAAITAKTKAIFVESLANPGGVVTDLEAIAKIADEAG
IPLIVDNTLATPYLINPIQWGATLVVHSTTKFLSGNGTSVGGVVIDSGSFDWGKSGKFPA
LSEPDAGYHGLKFQETFGHLAFTIHGHAVGLRDLGPSQAPLNAFLTLNGIETLPLRMQRH
SDSALKVAQFLESHPAVGWVSYAGLESSKYNALAKKYLPKGAGAVLTFGVKGGFDTGVKV
VENVQLLSHLANIGDSRSLIIHPSSTTHRQLSPEGQAQAGAGPDVLRLSIGLESVEDIIA
DLDRALSAA