Protein Info for AZOBR_RS06560 in Azospirillum brasilense Sp245

Annotation: acetolactate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00118: acetolactate synthase, large subunit, biosynthetic type
  • amino acids 6 to 568 (563 residues), 796.6 bits, E=5.7e-244
• PF02776: TPP_enzyme_N
  • amino acids 7 to 121 (115 residues), 138.8 bits, E=1.2e-44
• PF00205: TPP_enzyme_M
  • amino acids 196 to 332 (137 residues), 159.4 bits, E=6.2e-51
• PF02775: TPP_enzyme_C
  • amino acids 398 to 546 (149 residues), 174.2 bits, E=2.4e-55

Best Hits

Swiss-Prot: 48% identical to ILVI_BUCBP: Acetolactate synthase large subunit (ilvI) from Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)

KEGG orthology group: K01652, acetolactate synthase I/II/III large subunit [EC: 2.2.1.6] (inferred from 93% identity to azl:AZL_021740)

MetaCyc: 55% identical to acetohydroxyacid synthase subunit B (Cupriavidus necator H16)
Acetolactate synthase. [EC: 2.2.1.6]; 2.2.1.6 [EC: 2.2.1.6]

Predicted SEED Role

"Acetolactate synthase large subunit (EC 2.2.1.6)" in subsystem Acetoin, butanediol metabolism or Branched-Chain Amino Acid Biosynthesis (EC 2.2.1.6)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-valine biosynthesis (4/4 steps found)
• pyruvate fermentation to (R)-acetoin I (3/3 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• superpathway of (R,R)-butanediol biosynthesis (4/5 steps found)
• L-isoleucine biosynthesis II (6/8 steps found)
• superpathway of L-threonine metabolism (13/18 steps found)
• pyruvate fermentation to (S)-acetoin (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• pyruvate fermentation to (R)-acetoin II (1/2 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• superpathway of 2,3-butanediol biosynthesis (3/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.6

Use Curated BLAST to search for 2.2.1.6

Sequence Analysis Tools

See G8AKG2 at UniProt or InterPro

Protein Sequence (587 amino acids)

>AZOBR_RS06560 acetolactate synthase (Azospirillum brasilense Sp245)
MPEQKLTGAQIVIKALKDQGVDIIFGYPGGAVLPIYDAIFQQNDIKHILVRHEQAAVHAA
EGYARSTGKVGCVLVTSGPGATNAVTGLLDALCDSIPLVCLSGQVPTHLIGNDAFQEADT
TGITRPCTKHNYLVKDVNQLARTMHEAFYVARSGRPGPVLVDIPKDVQFADGTYIPPTEV
KHKTYRPQVKPEIARVEEAIELIATAKRPIFYTGGGVVNAGPIAAKLLTQFVKMTGFPIT
STLMGLGAFPASDPQWLGMLGMHGTYEANLAMYNCDVMINIGARFDDRVTGKLSEFAPGS
KKIHVDIDPSSINKNVAVDIPIVGDAGAVLEDMIRIWKARQKRADQTALKEWWGQVEGWR
ARNCLNYNRTEAVIKPQYALERLREALRGKNHYVTTEVGQHQMWAAQFLPFDEPNRWMTS
GGLGTMGYGLPAAIGAQLAHPDAIVVDVSGEASFLMNMQEIGTAVQYRAPVKIFILNNKY
MGMVRQWQELLHGSRYSNSYSEALPDFVKLAESWGCVGLRATTVAEVDEVIEKMLAVTDR
PCIIDIAVDPKENCFPMIPGGKAHNEILFGPEDSPSDATPEDGMVLV