Protein Info for AZOBR_RS05755 in Azospirillum brasilense Sp245

Updated annotation (from data): Butyryl-CoA dehydrogenase (EC 1.3.8.1)
Rationale: Specifically important for: Sodium butyrate. KEGG's annotation of azl:AZL_005040 has been updated to 3-(methylthio)propanoyl-CoA dehydrogenase [EC:1.3.8.-], which is questionable; phenotype indicates that butyryl-CoA is a substrate (SEED_correct)
Original annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 599 PF12418: AcylCoA_DH_N" amino acids 4 to 28 (25 residues), 33 bits, see alignment (E = 1.5e-11) PF02771: Acyl-CoA_dh_N" amino acids 42 to 157 (116 residues), 42.6 bits, see alignment E=2.2e-14 PF02770: Acyl-CoA_dh_M" amino acids 162 to 269 (108 residues), 52.9 bits, see alignment E=1e-17 PF00441: Acyl-CoA_dh_1" amino acids 284 to 450 (167 residues), 96 bits, see alignment E=7.6e-31 PF08028: Acyl-CoA_dh_2" amino acids 300 to 443 (144 residues), 33.6 bits, see alignment E=1.3e-11 PF12806: Acyl-CoA_dh_C" amino acids 466 to 594 (129 residues), 101.6 bits, see alignment E=1.1e-32

Best Hits

KEGG orthology group: None (inferred from 86% identity to azl:AZL_005040)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.1 or 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AJX1 at UniProt or InterPro

Protein Sequence (599 amino acids)

>AZOBR_RS05755 Butyryl-CoA dehydrogenase (EC 1.3.8.1) (Azospirillum brasilense Sp245)
MIPYTAPVDDLRFVLNEVVGLETVAALPNCDSAAPDLVDAVLEEAGKFASGVLAPLNRVG
DKEGSVLENGVVRTPTGWKDAYSQFTESGWNSLPFEPEYGGQGLPWTVAFAVNEMWQAAN
LSFGLCPLLTQGAVDLLTEHASAEQKALYLPKMIAGEWNGTMNLTEPQAGSDLAAVRTRA
VRADDGTYRITGQKIFITYGEHDLTDNIVHLVLARLPDAPPGIKGISLFIVPKFLPNADG
TPGARNDLRCASLEHKLGIMASPTAVMAFGDDGGAVGFLVGQENRGIEYMFTMMNNARLG
VGIQGVAIAERAYQQARDYAKGRVQSKDLADPKGSGVAIIRHPDVRRMLLDMRAKTEAAR
ALALYAGTQLDVSRHHEDATVRAAATARVDILTPIVKAWSTDIGCDVASTGVQIHGGMGF
IEETGAAQHYRDARITPIYEGTNGIHANDLTFRKTGRDGGESARTFIAEMRATVAELESI
PGDDMEAIRTNLSAGLDALEQAVAWVVKTQADGDLRAAAAGAVNYLKLWGTVAGGWMLAR
SAAKALEGLRQPGANAPFLEAKLVTARFYAEQILAQGPGLLLPILTAHRTVMALSEDQF