Protein Info for AZOBR_RS03470 in Azospirillum brasilense Sp245

Annotation: succinyl-diaminopimelate desuccinylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 TIGR01246: succinyl-diaminopimelate desuccinylase" amino acids 17 to 391 (375 residues), 420.3 bits, see alignment E=3.4e-130 PF01546: Peptidase_M20" amino acids 78 to 390 (313 residues), 153.6 bits, see alignment E=7e-49 PF07687: M20_dimer" amino acids 195 to 299 (105 residues), 77.7 bits, see alignment E=6.2e-26

Best Hits

Swiss-Prot: 66% identical to DAPE_RHOCS: Succinyl-diaminopimelate desuccinylase (dapE) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K01439, succinyl-diaminopimelate desuccinylase [EC: 3.5.1.18] (inferred from 75% identity to azl:AZL_005080)

Predicted SEED Role

"N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway (EC 3.5.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.18

Use Curated BLAST to search for 3.5.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AIA6 at UniProt or InterPro

Protein Sequence (397 amino acids)

>AZOBR_RS03470 succinyl-diaminopimelate desuccinylase (Azospirillum brasilense Sp245)
MSTAAAAPSFDPCDPVAVAQALIRCPSVTPEDGGALGVLEAALAPLGFVCHRLRFEEEGT
APVDNLYARLGTEGPNFCFAGHTDVVPPGELKGWSIDPFAAEIHNGRLYGRGAVDMKGAI
AAFVAAAARHVKAVKETGDAPKGSISLLITGDEEGVAINGTRKVLDWLQAKGEKLDACVV
GEPTNPKALGDMIKIGRRGSMTAFLTVHGAQGHVAYPHLADNPLPRLARMLTAITAEPLD
AGTPHFQPSNLELTTIDVGNPAHNVIPAQGKATLNIRFNDLHTADTLLAWLRERLDGVGG
SYELDHFVSGDSFLTPPGPLTELVADAAERVTGRRPEYSTTGGTSDARFIKNFCPVVEFG
LVGQTMHKVDEHTALDDLTRLTDIYGEVLRNVFDRIG