Protein Info for AZOBR_RS01145 in Azospirillum brasilense Sp245

Annotation: Crp/Fnr family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 245 PF00027: cNMP_binding" amino acids 51 to 133 (83 residues), 69.4 bits, see alignment E=3.3e-23 PF13545: HTH_Crp_2" amino acids 166 to 238 (73 residues), 63.1 bits, see alignment E=2.9e-21 PF00325: Crp" amino acids 192 to 222 (31 residues), 48.5 bits, see alignment 8.4e-17

Best Hits

Swiss-Prot: 43% identical to FIXK_BRADU: Nitrogen fixation regulation protein FixK (fixK) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01420, CRP/FNR family transcriptional regulator, anaerobic regulatory protein (inferred from 90% identity to azl:AZL_003420)

Predicted SEED Role

"transcriptional regulator, Crp/Fnr family" in subsystem Oxidative stress

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AFB5 at UniProt or InterPro

Protein Sequence (245 amino acids)

>AZOBR_RS01145 Crp/Fnr family transcriptional regulator (Azospirillum brasilense Sp245)
MPPFDMGRAREKGAASEMNPCGACPVRSLTVCAALDPEELRRLADILQTSRVEAGQTLFS
EGDAADALYNVTAGTVKLYKLLPDGRRQITGFLVTGDFLGLAVNESYAYTAETVTAATLC
RFPRKKIDALMDEFPKMQRRLFSMASNELAAAQDQMLLLGRKTAKEKICSFLLMLSQRAA
RRGHKENPVYVPMSRADIADYLGLTTETVSRTFTQLKTAGVISLQEGNKVLISDMDAIYD
MAEGC