Protein Info for AO356_26510 in Pseudomonas fluorescens FW300-N2C3

Annotation: asparagine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 554 (553 residues), 341.9 bits, E=4.1e-106
• PF13522: GATase_6
  • amino acids 31 to 159 (129 residues), 93.7 bits, E=2e-30
• PF13537: GATase_7
  • amino acids 50 to 164 (115 residues), 114.4 bits, E=7.1e-37
• PF02540: NAD_synthase
  • amino acids 241 to 374 (134 residues), 22.9 bits, E=8.7e-09
• PF00733: Asn_synthase
  • amino acids 244 to 604 (361 residues), 225.8 bits, E=2.6e-70

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See A0A0N9X1F0 at UniProt or InterPro

Protein Sequence (631 amino acids)

>AO356_26510 asparagine synthase (Pseudomonas fluorescens FW300-N2C3)
MCGFVVACHHQAPVPTVTLDAALDTLRHRGPDGRASWLSADGLCGIGFHRLAMVGAREQT
QPFHTDDVHVVVNGEIYQYAAQRQKLEAAGACFATDSDCEVVAHGYLQLGIDYVRELNGE
FAGVIWDARRRKLFAIRDRWGVKPLFYRLSDQGIHLASEIKALIALGAQPRWDHAALFQH
FFASIGPAQTLFEGIHQVPPGHVLEWSTQGYGVTPLLSAVDEAHDAARQHLASAPREWMP
RLVDGLRHAVQDRFQGDGPVACYLSGGVDSATVAALAARHGREGLSAFTVDFGAGTDDAT
QAAGIAASLGLRHEVVPVSEAQLVDHFADAVRHAETIGFNTIGAARWILGRVLASSGYKA
VLAGDGADELFAGYSFSSVERLCARSHNHARLMTQVLEGGRQGLAAELGQPLPLFDMDPD
RFGGQVPYLLASWNYQRSGLRPLLRTQFLEAFRHFNPYEVLMQQTGASAASGLRRSLHLW
QKSMFANHILVSERFDMAHGIETRYPFLDNRVTAVAAMLPDEWLVEGAENKRFLREAVAA
LTPEAARCAAKRPFEAPAVFAQPGDGPFRRYLREVLHDSSVKHSGIFDVQELLRLEARLP
SLSGKMAQRVDSLLTMALSFFVLQQCFAVTE