Protein Info for AO356_25875 in Pseudomonas fluorescens FW300-N2C3

Annotation: multidrug transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1031 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 335 to 353 (19 residues), see Phobius details amino acids 360 to 381 (22 residues), see Phobius details amino acids 388 to 410 (23 residues), see Phobius details amino acids 431 to 451 (21 residues), see Phobius details amino acids 463 to 485 (23 residues), see Phobius details amino acids 502 to 518 (17 residues), see Phobius details amino acids 530 to 549 (20 residues), see Phobius details amino acids 855 to 877 (23 residues), see Phobius details amino acids 884 to 903 (20 residues), see Phobius details amino acids 908 to 928 (21 residues), see Phobius details amino acids 959 to 978 (20 residues), see Phobius details amino acids 989 to 1014 (26 residues), see Phobius details PF00873: ACR_tran" amino acids 4 to 1014 (1011 residues), 1122.6 bits, see alignment E=0

Best Hits

Swiss-Prot: 66% identical to MDTB_ECOBW: Multidrug resistance protein MdtB (mdtB) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K07788, RND superfamily, multidrug transport protein MdtB (inferred from 80% identity to avn:Avin_28810)

MetaCyc: 66% identical to multidrug efflux pump RND permease subunit MdtB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-353; TRANS-RXN-92

Predicted SEED Role

"Acriflavin resistance plasma membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X4D2 at UniProt or InterPro

Protein Sequence (1031 amino acids)

>AO356_25875 multidrug transporter (Pseudomonas fluorescens FW300-N2C3)
MNLSRLFILRPVATTLSMLAIILAGLIAYRLLPVSALPQVDYPTIRVMTLYPGASPDVMT
SAVTAPLERQFGQMPGLTQMASTSSGGASVITLRFNLDINMDVAEQQVQAAINAATNLLP
KDLPAPPVYNKVNPADTPVLTLAITSKTMLLPKLNDLVDTRMAQKIAQISGVGMVTIAGG
QRQAVRIKVNPEALAANGLNLADVRTLIGASNVNQPKGNFDGPTRVSMLDANDQLTSPKD
YAELILAYANGSPLRLKDVAQIVDGAENERLAAWANENQAVLLNIQRQPGANVIEVVDRI
KALLPSITDNLPAGLDVTVLTDRTQTIRASVTDVQHELLIAIALVVMVTFLFLRRVSATI
IPSVAVPLSLIGTFGVMYLAGFSINNLTLMALTIATGFVVDDAIVMLENIARFIEEGDSP
LQAALKGAKQIGFTLISLTLSLIAVLIPLLFMADVVGRLFREFAITLAVAILISLVVSLT
LTPMMCARLLKREPKEAEQGRFYRASGAWIDWLIAAYGRKLQWVLKHQPLTLLVAVGSLV
LTVVLYLAVPKGFFPVQDTGVIQGISEAPQSISFAAMSERQQQLAKVILADPAVQSLSSY
IGVDGDNATLNSGRLLINLKPHSERDDSATEIIARLQPQLDRLVGIRLFMQPVQDLTIED
RVSRTQYQFSLSSPDAELLSLWSGRLVEALGRQPELTDVASDLQDKGLQVYLVIDRDAAS
RLGVSVSNITDALYDAFGQRQISTIYTQASQYRVVLQAQAGERIGPKALDQIHVKTTDGG
QVRLSSLARVEERQAQLAIAHIGQFPAVMMSFNLAPGVALGHAVDMIEKVQQDIGMPVGV
QTEFQGAAEAFQASLSSTLLLILAAVVTMYIVLGVLYESYIHPITILSTLPSAAIGALLA
LILSGNDLGMIAIIGIILLIGIVKKNAIMMIDFALDAERNQGMDPQTAIYQAALLRFRPI
LMTTLAALFGAIPLMFATGSGAELRQPLGLVMVGGLLVSQVLTLFTTPVIYLYFDRLGRR
FARPEGKEVRV