Protein Info for AO356_17710 in Pseudomonas fluorescens FW300-N2C3

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 763 PF08447: PAS_3" amino acids 212 to 297 (86 residues), 51.4 bits, see alignment E=2.7e-17 PF08448: PAS_4" amino acids 219 to 305 (87 residues), 33 bits, see alignment E=1.5e-11 TIGR00229: PAS domain S-box protein" amino acids 220 to 310 (91 residues), 38.5 bits, see alignment E=1.2e-13 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 314 to 475 (162 residues), 140 bits, see alignment E=6.2e-45 PF00990: GGDEF" amino acids 316 to 472 (157 residues), 154.5 bits, see alignment E=5.3e-49 PF00563: EAL" amino acids 492 to 727 (236 residues), 196.5 bits, see alignment E=1.2e-61

Best Hits

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a4512)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WAB9 at UniProt or InterPro

Protein Sequence (763 amino acids)

>AO356_17710 diguanylate cyclase (Pseudomonas fluorescens FW300-N2C3)
MSLPLDKTNRRILIVDDTPTIHEDFRKILSPQMNAEDNLSSAEEALFGAPVAVAAMSFVL
DSAFQGMEALNKVEKALAQGQPFAMAFIDMRMPPGWDGLETIERLWRADPKLQVALCTAY
SDYSWEDISERLELGDRLLILKKPFDAIEIRQMASALTVKWQMTEDAALKMDMLERAVEE
RTRELADANIIVQNSPTILYRLRGEPPFALMYISHNITKFGHSAAQLINAPDWAQTLIHP
DDQDKVREAMVRVLDRDTAGASIEFRMRTGDDTFRWVENRYIPVRNDQGLLLEVEGIILD
ITERKLAEEKIALLARTDGLTGLANRATMIERLHQAFAAARRGAAPFAMFYLDLDHFKRI
NDTLGHPIGDLLLQEVAARIKSCTRENDVVARLGGDEFALLQLDVHEATHCAALAGKIRD
ALVAPYSLDGNDVRISVSIGIALYTPQSLSADSLMAQSDMALYRSKEKGRNQYHFHNEEI
NQEVTDRVALANDLRQAIENQELHLHYLPEVDLGSGKILGMGVQVRWNHPQRGWLEPAAF
MPAAEKTGIIIGLGHWVLDQACQQMRQWRDQGMAPPVIAIDLSLTQLKTGPELIYDVLRT
TARWELAPWDLRFDVTEATLAQTKWTHNDVLPRLCELGVQIAIDDFGTEYSSFDYLKTYR
VNHLKLAQRFLDSANADPENATTLRAIINFARDVGIGIIAEGVETQEQRTTLMSSGGAMQ
AQGHYFSTVVDSGQAAELLKAGTIIPATDHWTRPASQLSESNP