Protein Info for AO356_16055 in Pseudomonas fluorescens FW300-N2C3

Annotation: pantoate--beta-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 91% identical to PANC_PSEPF: Pantothenate synthetase (panC) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01918, pantoate--beta-alanine ligase [EC: 6.3.2.1] (inferred from 98% identity to pba:PSEBR_a4817)

MetaCyc: 55% identical to pantothenate synthetase (Escherichia coli K-12 substr. MG1655)
Pantoate--beta-alanine ligase. [EC: 6.3.2.1]

Predicted SEED Role

"Pantoate--beta-alanine ligase (EC 6.3.2.1)" in subsystem Coenzyme A Biosynthesis (EC 6.3.2.1)

MetaCyc Pathways

superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
superpathway of coenzyme A biosynthesis I (bacteria) (8/9 steps found)
phosphopantothenate biosynthesis I (4/4 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X0I3 at UniProt or InterPro

Protein Sequence (286 amino acids)

>AO356_16055 pantoate--beta-alanine ligase (Pseudomonas fluorescens FW300-N2C3)
MNTVKTVRELRAAVARARGEGKRIAFVPTMGNLHSGHVALVTKAAQRADFVVASIFVNPL
QFGAGEDLDKYPRTLAADQEKLLQAGCHLLFAPSVEEMYPDGMAGQTRVSVPQLSEGLCG
ASRPGHFEGVATVVSKLFNMVQPDLAVFGQKDFQQLAVIRALVHDLNMPIQIIGEPTVRA
EDGLALSSRNGFLSPDQRAVAPVVYRVLSQIAESIKQGQRDFPALIGEQLKQLEAAGLRP
DYLEIRHAKTLRPALSEDRDLVILVAAFLGTTRLIDNLHLDLDTPA