Protein Info for AO356_14165 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): Acyl-CoA dehydrogenase (EC 1.3.8.7)
Rationale: Specifically important for: Tween 20. tween 20 hydrolyzes to a mix of C12, C14, and C16 fatty acids; this is probably part of beta oxidation (SEED_correct)
Original annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 PF12418: AcylCoA_DH_N" amino acids 3 to 35 (33 residues), 43.8 bits, see alignment (E = 5.2e-15) PF02771: Acyl-CoA_dh_N" amino acids 41 to 158 (118 residues), 42.6 bits, see alignment E=1.8e-14 PF02770: Acyl-CoA_dh_M" amino acids 163 to 271 (109 residues), 50.3 bits, see alignment E=5.7e-17 PF00441: Acyl-CoA_dh_1" amino acids 282 to 450 (169 residues), 67 bits, see alignment E=5.3e-22 PF12806: Acyl-CoA_dh_C" amino acids 467 to 592 (126 residues), 132 bits, see alignment E=3.4e-42

Best Hits

KEGG orthology group: None (inferred from 100% identity to pba:PSEBR_a5175)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WIE7 at UniProt or InterPro

Protein Sequence (601 amino acids)

>AO356_14165 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Pseudomonas fluorescens FW300-N2C3)
MPDYKAPLRDIRFVRDELLGYEAHYQSLPACQDATPDMVDAILEEGAKFCEQVLAPLNRV
GDIEGCTWSESGVKTPAGFKEAYKQFVEGGWPSLAHDVEHGGQGLPESLGLAVSEMVGEA
NWSWGMYPGLSHGAMNTISEHGTPEQQEAYLTKLVSGEWTGTMCLTEPHCGTDLGMLRTK
AEPQADGSYKVSGTKIFISAGEHDMADNIVHIVLARLPDAPAGTKGISLFIVPKFLPNAD
GSIGQRNAVSCGSLEHKMGIHGNATCVMNFDAATGYLIGPANKGLNCMFTFMNTARLGTA
LQGLAHAEIGFQGGLKYARDRLQMRSLTGPKAPDKAADPIIVHPDVRRMLLTMKAFAEGN
RAMVYFTAKQVDIVKYGVDEEEKKKADALLAFMTPIAKAFMTEVGFESANHGVQIYGGHG
FIAEWGMEQNVRDSRISMLYEGTTGIQALDLLGRKVLMTQGEALKGFTKIVHKFCQSNEG
NEAVKEFVEPLAALNKEWGELTMKVGMAAMKDREEVGAASVDYLMYSGYACLAYFWADMA
RLAAEKLAAGTTEEAFYTAKLQTARFYFQRILPRTRTHVATMLSGANNLMDMKEEDFALG
Y