Protein Info for AO356_12220 in Pseudomonas fluorescens FW300-N2C3

Annotation: type IV secretion protein Rhs

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1338 transmembrane" amino acids 22 to 46 (25 residues), see Phobius details amino acids 52 to 74 (23 residues), see Phobius details amino acids 265 to 287 (23 residues), see Phobius details PF05488: PAAR_motif" amino acids 174 to 219 (46 residues), 38.2 bits, see alignment (E = 2.7e-13) PF20148: DUF6531" amino acids 297 to 375 (79 residues), 70 bits, see alignment 3.5e-23 PF05593: RHS_repeat" amino acids 489 to 525 (37 residues), 24.9 bits, see alignment (E = 4.2e-09) amino acids 578 to 614 (37 residues), 24.2 bits, see alignment (E = 7.2e-09) amino acids 604 to 635 (32 residues), 33.3 bits, see alignment (E = 1e-11) amino acids 652 to 678 (27 residues), 22.3 bits, see alignment (E = 2.9e-08) amino acids 663 to 699 (37 residues), 34.5 bits, see alignment (E = 4.2e-12) amino acids 705 to 741 (37 residues), 34.9 bits, see alignment (E = 3.1e-12) amino acids 732 to 763 (32 residues), 30.3 bits, see alignment (E = 8.8e-11) amino acids 747 to 784 (38 residues), 32.9 bits, see alignment (E = 1.3e-11) amino acids 769 to 804 (36 residues), 26 bits, see alignment (E = 1.9e-09) amino acids 790 to 825 (36 residues), 32 bits, see alignment (E = 2.5e-11) amino acids 810 to 846 (37 residues), 29.6 bits, see alignment (E = 1.5e-10) amino acids 890 to 919 (30 residues), 27 bits, see alignment (E = 9.3e-10) amino acids 934 to 969 (36 residues), 22.7 bits, see alignment (E = 2.1e-08) TIGR01643: YD repeat (two copies)" amino acids 578 to 615 (38 residues), 26.3 bits, see alignment (E = 7.4e-10) amino acids 599 to 639 (41 residues), 33.9 bits, see alignment (E = 3e-12) amino acids 651 to 682 (32 residues), 24.9 bits, see alignment (E = 2.2e-09) amino acids 663 to 703 (41 residues), 29.1 bits, see alignment (E = 1e-10) amino acids 705 to 745 (41 residues), 31.5 bits, see alignment (E = 1.8e-11) amino acids 747 to 787 (41 residues), 35.9 bits, see alignment (E = 7.3e-13) amino acids 789 to 818 (30 residues), 19.5 bits, see alignment (E = 1.1e-07) amino acids 814 to 850 (37 residues), 24.6 bits, see alignment (E = 2.7e-09) amino acids 890 to 922 (33 residues), 27.8 bits, see alignment (E = 2.6e-10) amino acids 934 to 969 (36 residues), 17.3 bits, see alignment (E = 5.3e-07) amino acids 1145 to 1176 (32 residues), 24.3 bits, see alignment (E = 3.4e-09) PF03527: RHS" amino acids 1231 to 1267 (37 residues), 55.5 bits, see alignment (E = 8.1e-19) TIGR03696: RHS repeat-associated core domain" amino acids 1258 to 1337 (80 residues), 86.5 bits, see alignment 1.4e-28

Best Hits

KEGG orthology group: None (inferred from 94% identity to pba:PSEBR_a5571)

Predicted SEED Role

"Rhs-family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1338 amino acids)

>AO356_12220 type IV secretion protein Rhs (Pseudomonas fluorescens FW300-N2C3)
MSDALWAARLGDALNHTSMMADILGGVLEVAANIAITALATAAVVAATGITVVTGGLGCF
VLGLVVGTIVGLAMSKTGADKGLSNLCEGIGNALFPPTVQANILTGSKDTFTNNIPSARA
AGAVVSHVAPAGTELEMPEPEEEPSYLDMAGSFFSQMWRPTVASPAPGTETRPEDLVICT
KHPPMPPQFMAEGSEKVTINGQPAVRSGDRSTCDATVVSAGLISPDVTIGGGSVVVREIR
SGKTPGVGLAVTALLMLKGGKGKFFSKLPCMLVGGAVSMAASSAANAAANAAMGSSNPVH
AATGAKVLGADEDLDFVLPGILPIDWQRIYNSRDERRDSLFGAGWSVAYEVQVEIQPHPE
GGETLVYTDEQGRPIDMGSIPLGGAVFSAGEGLAVRRHVNGQLLIESEDGLYRLFEPSPA
NPSHLRLNQLGDRNDNRIHLDYDDAGRLVRLRDTFDLVHVELIRAGERVTHIERLYPDQT
REVLVSYAYDAANTLAEVRDASGQVQRRFRYDAGQRMVEHQLPTGLHCFYEWALIDDQEW
RVVRHWTDEGDAYQFDYDLEAGTTRISDSLQRVSTRQWNSQHQITHYTDNLGQTWLFEWN
DERQLLSATDPQGGQYSFSYDEAGNLLSETDPLGRSESTLWLEHWALPLVDTNPAGHSWK
YRYDQRGNCTAEIDPLGHVTRYRHDAHGQVVEIIDATGKSKKLRWNPFGQLVEHVDCSGY
PTRFSYDARGYLQTITDALGERTQFSYDAQGRLLGSQLPDGRTERYQRDTAGQLTGYTDP
AGHTTLYQHNRRGQVRQRTDAQGRQVQFVYDSFGRLQALTNENGESYRFAWDAGDRLAEQ
QDLDGSAKRYDYDRLDNVAAVTAIPAPYGTGLAIIPETPPATIVHRLERDAAGRLIAKVT
DDGRTEYSYDPLDQLTAVTFTDLQGNAQTLSFAYDALGQLLAENSAAGSLQHRYDELGNR
IQSQLPDGRWVNRLYYGSGHLHQINLDGQVISDFERDRLHREVLRTQGQISTRSEYDRSG
RLRTRQRRHSSQPSLLPAAVQQHFEYDPANNLIGKLDQQPTTQQRQLLHYDATGRIIASQ
DSLHGQRETFTYDAAANLLDGPAPGAGLVVHNKLLTYQDKRYRYDAFGRMIEKRSAKLGV
QHFTYDAESRLIEVRNDDGSVVNMAYDPLGRRIEKTEHDNHGYPLGETRFTWDGLRLLQE
HRHQQTSLYLYEDDGYEPLARVDGSGPLQKIRYYHNDLNGLPEQLTEADGHNVWQATYRV
WGNTLEEVREPYYIEEQNLRFQGQYLDRETGLHFNTFRFYDPDVGRFTTPDPIGLAGGIN
FYAYAPNPAGYADPLGLS