Protein Info for AO356_11730 in Pseudomonas fluorescens FW300-N2C3

Annotation: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 451 (448 residues), 658.2 bits, E=3.3e-202
• PF00483: NTP_transferase
  • amino acids 6 to 194 (189 residues), 51.9 bits, E=2e-17
• PF12804: NTP_transf_3
  • amino acids 6 to 123 (118 residues), 78.6 bits, E=1.6e-25
• PF00132: Hexapep
  • amino acids 263 to 296 (34 residues), 29.4 bits, 1.2e-10
• PF14602: Hexapep_2
  • amino acids 281 to 311 (31 residues), 16.3 bits, see alignment (E = 1.6e-06)
  • amino acids 394 to 426 (33 residues), 18.9 bits, see alignment (E = 2.6e-07)

Best Hits

Swiss-Prot: 91% identical to GLMU_PSEPF: Bifunctional protein GlmU (glmU) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 95% identity to pba:PSEBR_a5650)

MetaCyc: 55% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• peptidoglycan recycling I (11/14 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (13/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See A0A0N9W7T0 at UniProt or InterPro

Protein Sequence (455 amino acids)

>AO356_11730 bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase (Pseudomonas fluorescens FW300-N2C3)
MSLEIVILAAGQGTRMRSALPKVLHPVAGNSMLGHVIHSARQLDPQRIHVVIGHGADAVR
ERLAADDLNFVLQDKQLGTGHAVAQAVPFITADTVLILYGDVPLIEVETLQRLLKHAAPQ
QLGLLTVELDDPTGYGRIVRNADGQVTAIVEQKDANEAQRAITEGNTGILAVPAERLGDW
MSRLSNNNAQGEYYLTDVIAMAVADGLVVATEQPLDAMEVQGANDRRQLAELERHYQLRA
ARRLMAQGVTLRDPSRFDVRGEVSVGRDVVIDINVILEGKVVIEDDVVIGPNCVIKDSTL
RKGVVIKANSHLDGAVMGEGSDAGPFARLRPGSVLEARAHVGNFVELKNAHLGEDAKAGH
LTYLGDAEVGARTNIGAGTITCNYDGANKWKTVMGDDVFIGSNNSLVAPVDISSGATTAA
GSTLTQNVDNGQLAVARARQRNIDGWKRPEKIKKP