Protein Info for AO356_09415 in Pseudomonas fluorescens FW300-N2C3

Annotation: pilus assembly protein PilM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 TIGR01175: type IV pilus assembly protein PilM" amino acids 10 to 353 (344 residues), 465 bits, see alignment E=7.3e-144 PF11104: PilM_2" amino acids 14 to 353 (340 residues), 465.7 bits, see alignment E=1.2e-143 PF14450: FtsA" amino acids 196 to 323 (128 residues), 47.2 bits, see alignment E=4.2e-16

Best Hits

KEGG orthology group: K02662, type IV pilus assembly protein PilM (inferred from 98% identity to pba:PSEBR_a422)

Predicted SEED Role

"Type IV pilus biogenesis protein PilM" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WVS4 at UniProt or InterPro

Protein Sequence (354 amino acids)

>AO356_09415 pilus assembly protein PilM (Pseudomonas fluorescens FW300-N2C3)
VLRLFNKKAHALLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVIEKNIAELEGV
GQALSRLLAKAKTPLRSVAVAVAGSAVITKIIEMDAGLSDDDMENQLKIEADQYIPYPLD
EVAIDFEVVGVSPRSAERVEVLLAACRKENVEVREAALALAGLTARVVDVEAYALERAFG
LLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASV
AGLDRLIEQRLGTPTQVANPFTNMALSSKVNAGALASDAPALMIACGLALRSFD