Protein Info for AO356_03000 in Pseudomonas fluorescens FW300-N2C3

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 PF00849: PseudoU_synth_2" amino acids 95 to 242 (148 residues), 100.3 bits, see alignment E=6.5e-33

Best Hits

KEGG orthology group: None (inferred from 94% identity to pba:PSEBR_a1646)

Predicted SEED Role

"Similar to ribosomal large subunit pseudouridine synthase A" in subsystem Ribosome biogenesis bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W3I7 at UniProt or InterPro

Protein Sequence (296 amino acids)

>AO356_03000 pseudouridine synthase (Pseudomonas fluorescens FW300-N2C3)
MSGSPFSAADHQASTLYLPPGSWPTVLECLCEHFSAISREQWLDRIARGRVLDGEGKAIH
VDLPYREGLRIHYFREVPDEKPIPVVESILYADEHLVVADKPHFLPVTPAGEYVEQTLLR
RLIRRLDNPHLVPLHRIDRHTAGLVLFSANPHTRSAYQSLFPTRQIEKRYQAIARALPGL
TFPRVHRSRMIDGEPFFRMQEGPGEPNTETAIEVLERHGDLWRYGLSPVTGKKHQLRVHM
NALGAGICNDPFYPDVLRDAEDDYANPLKLLAQGLRFIDPVTGQERVFQSEITLQW