Protein Info for AO356_02930 in Pseudomonas fluorescens FW300-N2C3

Annotation: 3-methylcrotonyl-CoA carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 656 PF00289: Biotin_carb_N" amino acids 4 to 112 (109 residues), 152 bits, see alignment E=3.1e-48 PF02786: CPSase_L_D2" amino acids 118 to 324 (207 residues), 230 bits, see alignment E=8.9e-72 PF02222: ATP-grasp" amino acids 135 to 294 (160 residues), 27.9 bits, see alignment E=6.7e-10 PF07478: Dala_Dala_lig_C" amino acids 141 to 293 (153 residues), 36.9 bits, see alignment E=1.1e-12 PF02785: Biotin_carb_C" amino acids 338 to 444 (107 residues), 113 bits, see alignment E=3e-36 PF00364: Biotin_lipoyl" amino acids 588 to 652 (65 residues), 53.2 bits, see alignment E=8.7e-18 PF13533: Biotin_lipoyl_2" amino acids 589 to 622 (34 residues), 24.9 bits, see alignment (E = 5.7e-09)

Best Hits

KEGG orthology group: K13777, geranyl-CoA carboxylase alpha subunit [EC: 6.4.1.5] (inferred from 88% identity to pba:PSEBR_a1659)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4, 6.4.1.5

Use Curated BLAST to search for 6.4.1.4 or 6.4.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WT97 at UniProt or InterPro

Protein Sequence (656 amino acids)

>AO356_02930 3-methylcrotonyl-CoA carboxylase (Pseudomonas fluorescens FW300-N2C3)
MPAFSKILIANRGEIACRIQRTAQALGYRTVAVFSEADADALHVRMADEAVLIGAAPVQQ
SYLDPRVILEAARRSGADAIHPGYGFLSENARFAHACAQAGLVFIGPSPEAIELMGSKRR
AKIAMIQAGVPCIAGYQGAAQDDDTLQREAGRIGYPLMIKASAGGGGRGMRLVQRAEDLL
EQLRSARSEALHGFGSDELILEQALIDPRHVEVQIFGDHHGQLIHLGERDCSIQRRHQKI
IEEAPCPVMTADLRRAMGEAALTAGHAVNYVGAGTVEFLLAADGRFYFLEMNTRLQVEHP
VTESITGLDLVAWQLDVATGKPLPLCQEQVTLSGHALQVRLYAEDPAADFLPQTGCLVGW
EPPIRDGVRVDHGLLQGQAITPFYDSMLGKLIVHGATRDEARRKLLRAVEDCVLLGVQSN
QRLLAGLLAHPRFIDGDFNTGFISQHFANHAALLPLEPSVEQLAIATAAFYQASQARHAP
GLGGWRNSIGTALHYRIGVGERDWVLSLETETDGTLNIRSDGRLIELRLLDVDAQGATLV
IDGIGQRHAWHLEGHDLWLSTRPGGLHLQDRTLAPVASKASAGASTLLAPMDGAIVEVLV
SEGSPVRQGQLLMVLEAMKMEHPLKAGSDGVVRQLQATRGDQVRKRQVLLRIETAH