Protein Info for AO356_02605 in Pseudomonas fluorescens FW300-N2C3

Annotation: 50S rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 756 PF02926: THUMP" amino acids 42 to 156 (115 residues), 39 bits, see alignment E=2.2e-13 PF01170: UPF0020" amino acids 165 to 380 (216 residues), 141.8 bits, see alignment E=5.8e-45 PF10672: Methyltrans_SAM" amino acids 528 to 708 (181 residues), 72.1 bits, see alignment E=1.1e-23 PF03602: Cons_hypoth95" amino acids 590 to 679 (90 residues), 31.2 bits, see alignment E=4.3e-11

Best Hits

Swiss-Prot: 95% identical to RLMKL_PSEPF: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 100% identity to pba:PSEBR_a1694)

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VXW2 at UniProt or InterPro

Protein Sequence (756 amino acids)

>AO356_02605 50S rRNA methyltransferase (Pseudomonas fluorescens FW300-N2C3)
MSDRFELFLTCPKGLEGLLIEEAVGLGLEEAREHTSAVRGMADMETAYRLCLWSRLANRV
LLVLKRFPMKDAEDLYHGVLDVDWQDHMLADGTLAVEFSGHGSGIDNTHFGALKVKDAIV
DKLRTPTGERPSIDKLNPDLRIHLRLDRGEAILSLDLSGHSLHQRGYRLQQGAAPLKENL
AAAILIRAGWPRIAAEGGALADPMCGVGTFLVEAGMIAADMAPNLRRQQWGFTAWLGHVP
ALWKKLHEEATARAAAGLAKPPLWIRGYEADPRLIQPGRNNVERAGLSEWIKIYQGEVAT
FEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGK
RMGIRSHKQYSFWNGALPCKLLLIKVLPDQFVTGERRTPEQRQAEREQAAYDQAPEVPQE
RQYNKNGNPIKPAPAPAPVVEQARLSEGGQMFANRLQKNLKLLSKWAKREGVDCYRVYDA
DMPEYSMAIDLYHDWVHVQEYVAPKSIDPEKASARLFDALAAIPQALNVDKSRVVIKRRE
RQSGTKQYERQSAQGKFTEVNEGGVKLLVNLTDYLDTGLFLDHRPMRLRIQKEAAGKRFL
NLFCYTATASVHAAKGGARSTTSVDLSKTYLDWARRNLSLNGFSDKNRLEQGDVMAWLEA
SRDEYDLIFIDPPTFSNSKRMEGVFDVQRDHVQLLDLAMARLAPGGVLYFSNNFRKFQLE
DNLGERYAVEEITAKTIDPDFARNGKIHRAWKIMAR