Protein Info for AO356_00020 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): ABC transporter for D-Sorbitol, permease component 2
Rationale: Specific phenotypes on D-Sorbitol; D-Sorbitol. (no phenotype on mannitol)
Original annotation: sugar ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 transmembrane" amino acids 26 to 51 (26 residues), see Phobius details amino acids 86 to 109 (24 residues), see Phobius details amino acids 119 to 139 (21 residues), see Phobius details amino acids 169 to 192 (24 residues), see Phobius details amino acids 227 to 247 (21 residues), see Phobius details amino acids 277 to 299 (23 residues), see Phobius details PF00528: BPD_transp_1" amino acids 99 to 303 (205 residues), 54.9 bits, see alignment E=5e-19

Best Hits

KEGG orthology group: None (inferred from 100% identity to pba:PSEBR_a2025)

MetaCyc: 87% identical to polyol ABC-type transporter permease component MtlF (Pseudomonas fluorescens)
7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]

Predicted SEED Role

"Various polyols ABC transporter, permease component 1" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WK88 at UniProt or InterPro

Protein Sequence (308 amino acids)

>AO356_00020 ABC transporter for D-Sorbitol, permease component 2 (Pseudomonas fluorescens FW300-N2C3)
MNTSIIRAQLAASAPSRARRLINPGWFLVSPSVALLLLWMIVPLAMTVYFSVIRYNLLNP
GENEFVGLENFAYFVTDSGFLPGALNTLILVGSVLLISVIFGVLIAALLEASEFFGRGIV
RVLLISPFFIMPTVGSLIFKNLIFHPVSGILAAVWKFFGAQPVDWLAHYPLFSIIVIVSW
QWLPFAILLLMTAMQSLDQEQKEAARLDGAGALAIFWHLTLPHLARPIAVVVMIETIFLL
SVFAEIFTTTNGGPGFASTNLAYLIYNQALVQFDVGMASAGGLIAVVIANIAAIVLVRMI
GKNLTDKA