Protein Info for AO353_29020 in Pseudomonas fluorescens FW300-N2E3
Annotation: amidohydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 49% identical to HIPO_CAMJE: Hippurate hydrolase (hipO) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 77% identity to pae:PA2922)MetaCyc: 42% identical to N-acetyl amino acid acetylase (Bacillus subtilis subtilis 168)
3.5.1.-
Predicted SEED Role
"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization
MetaCyc Pathways
- S-(2-succinyl)-L-cysteine degradation (2/4 steps found)
- S-benzyl-L-cysteine degradation (1/5 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.32
Use Curated BLAST to search for 3.5.1.32
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9WRW9 at UniProt or InterPro
Protein Sequence (389 amino acids)
>AO353_29020 amidohydrolase (Pseudomonas fluorescens FW300-N2E3) MNAGHVLPGIAALQDEMITLRQRIHAHPELGFEEFSTSELVAQCLTQWGYDVSTGVGKTG VVGTLKNGEGRSLGLRADMDALPIQEATGLPYASRIDGVMHACGHDGHTATLLAAAKYLA QTREFNGTLNLVFQPAEEGLGGAKKMLEDGLFERFPCDAIFAMHNIPGYPAGQLGFYSGP FMASADTVTIKVIGTGGHGAVPHKSVDPVVVCASIVIALQSIVSRNVNPQETAIITVGSI HAGNVSNVIPASAEMILSVRALTPEVRQLLERRITDLVNGQAASFGAQAEIDYYRCHPVL INDPEQTAFARQVAREWLGEGQLIEDLRPFTASEDFAFILEQCPGSYLVIGNGQGEGSCL LHNPGYDFNDACLPIGASYWVKLAEGFLR