Protein Info for AO353_29015 in Pseudomonas fluorescens FW300-N2E3

Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00497: SBP_bac_3" amino acids 29 to 255 (227 residues), 202 bits, see alignment E=8.9e-64

Best Hits

Swiss-Prot: 55% identical to HISJ_ECOLI: Histidine-binding periplasmic protein (hisJ) from Escherichia coli (strain K12)

KEGG orthology group: K10014, histidine transport system substrate-binding protein (inferred from 70% identity to pau:PA14_26240)

Predicted SEED Role

"Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1)" in subsystem Arginine and Ornithine Degradation (TC 3.A.1.3.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W1A4 at UniProt or InterPro

Protein Sequence (262 amino acids)

>AO353_29015 ABC transporter substrate-binding protein (Pseudomonas fluorescens FW300-N2E3)
MNKMIAAVSLVFLTASGLAGAADWSGKVLRLGIDPTYPPLEYKNQDGTLTGFGVDIADAL
CAELHAKCVWVESSWDGMIPGLLARKFDAIASSMTVTPKRQEQIAFSDKVSNAPARLVIK
RGSDLQPTVESLKGKRIGVEQGSTQEAFAKAVWGKQGVEILSYQNQDQVYADLVVGRLDA
SLQGSIQASYGFLNKPVGKDYEFAGGTLDNPEFFGVGDGIGVRKSDVELREDLNKALATI
LANGTYAKINKKYFDFDIYGAK