Protein Info for AO353_25190 in Pseudomonas fluorescens FW300-N2E3

Annotation: amino acid transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 transmembrane" amino acids 21 to 39 (19 residues), see Phobius details amino acids 43 to 66 (24 residues), see Phobius details amino acids 102 to 124 (23 residues), see Phobius details amino acids 130 to 152 (23 residues), see Phobius details amino acids 160 to 183 (24 residues), see Phobius details amino acids 205 to 227 (23 residues), see Phobius details amino acids 248 to 269 (22 residues), see Phobius details amino acids 281 to 305 (25 residues), see Phobius details amino acids 340 to 360 (21 residues), see Phobius details amino acids 366 to 392 (27 residues), see Phobius details amino acids 412 to 430 (19 residues), see Phobius details amino acids 436 to 454 (19 residues), see Phobius details PF00324: AA_permease" amino acids 22 to 459 (438 residues), 333.7 bits, see alignment E=1.8e-103 PF13520: AA_permease_2" amino acids 25 to 434 (410 residues), 134.4 bits, see alignment E=5.6e-43

Best Hits

Swiss-Prot: 58% identical to CYCA_ECOLI: D-serine/D-alanine/glycine transporter (cycA) from Escherichia coli (strain K12)

KEGG orthology group: K11737, D-serine/D-alanine/glycine transporter (inferred from 94% identity to pba:PSEBR_a2901)

MetaCyc: 58% identical to D-serine/alanine/glycine:H+symporter (Escherichia coli K-12 substr. MG1655)
RXN0-5130; RXN0-5201; RXN0-5202; RXN0-5203; TRANS-RXN-62A; TRANS-RXN-62B

Predicted SEED Role

"D-serine/D-alanine/glycine transporter" in subsystem Glycine and Serine Utilization or Pyruvate Alanine Serine Interconversions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VZ42 at UniProt or InterPro

Protein Sequence (471 amino acids)

>AO353_25190 amino acid transporter (Pseudomonas fluorescens FW300-N2E3)
MKSTPSERVEQPALQRTLSNRHIQLMAMGGAIGTGLFMGSGKIIALSGTSIILIYMIIGM
FVYFVMRAMGELLLSNLNFKTFADFTGAYLGPRAAFFLGWSYWLSWSVAVVGDAVVVGGF
FQYWFPDVPAWIPAVGMLMTLFALNVLTVRLFGEVEFWFAIIKIIAVVTLIGVSLVMIAS
SFVSPSGVTASLNHLLDKQAAFPNGLFGFFAGFQMAIFSFAGTELIGTAAAETRSPEKTL
PKAINSIPLRIILFYVLALACIIAVTSWQQVSPNKSPFVELFLVAGFPAAAGIVNFVVLT
SAASSANSGVFSSSRMLFGLASHDNAPGIFRHLSRNSVPLLSLAFTTLLMLVGVLLLFIV
PEVMTAFTIVSTVSAILVIFTWSTILASYIAYRKSRPDLHAKSVYKMPGGVPMAWFSLTF
LGFVLCLLALRPDTRIALLVMPGWFIWLAIAYQLTHSRKLRSAAGSANQYS