Protein Info for AO353_25170 in Pseudomonas fluorescens FW300-N2E3

Annotation: Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 630 PF01590: GAF" amino acids 77 to 204 (128 residues), 39.5 bits, see alignment E=2.3e-13 PF00158: Sigma54_activat" amino acids 324 to 484 (161 residues), 219.3 bits, see alignment E=7.9e-69 PF14532: Sigma54_activ_2" amino acids 336 to 491 (156 residues), 86.1 bits, see alignment E=8.3e-28 PF07728: AAA_5" amino acids 342 to 442 (101 residues), 24.1 bits, see alignment E=9.3e-09 PF02954: HTH_8" amino acids 584 to 617 (34 residues), 32.5 bits, see alignment (E = 1.7e-11)

Best Hits

KEGG orthology group: None (inferred from 84% identity to pfl:PFL_2168)

Predicted SEED Role

"Transcriptional activator of acetoin dehydrogenase operon AcoR" in subsystem Acetoin, butanediol metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VUA7 at UniProt or InterPro

Protein Sequence (630 amino acids)

>AO353_25170 Fis family transcriptional regulator (Pseudomonas fluorescens FW300-N2E3)
MLSAHSKAHVDCVSRVLKNAGHLPQLPVSDLILDSWRRSMEQHHLDPGSLQGPRILSQNV
LQECRERSELFLRIASEEVARLHGRVRDADYCVLLTDAQGQTIDYRVETSIRNDCRKAGL
YLGTCWSEGEEGTCGVAAVLTARTPVTVHKRDHFRAAFIGLTCSAAPVFDPKGELLGVLD
VSAVRSPDDRRSQHLIRQMVVQSAREIEQAFFMSSAQGYWVLRAHSSPGYVDSQPDYLLA
WDNDGCLQALNPAARQWLLHRFGQLPEHIGQVFDQQLLHRISDESLCLLHSQGGHTQLHG
RLSVPRRVNRNASRLALTPAELDPRLEESLRLAVRVKDRNLPVLIQGETGSGKEVFARQL
HQASQRRERPFVAVNCAAIPENLIESELFGYVAGAFTGASSKGMQGLLEQADGGTLFLDE
IGDMPLSLQTRLLRVLAEGEVAPLGASRRKAIDLQVVCASHRDLEGLVAAGEFREDLYFR
LNGARFQLPPLRERSDRLALINRILDEESTLCAEPMHLGGAALECLLGYRWPGNVRQLRH
VLRYACAVCDSNLIQVSHLPEQLQDHRLDGPGSGDTIDCCASPERQALLDALVRHRWKPT
AAAKSLGISRATLYRRVHQHGIDMPGRSQV