Protein Info for AO353_23435 in Pseudomonas fluorescens FW300-N2E3

Annotation: twin-arginine translocation pathway signal protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 722 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details PF10518: TAT_signal" amino acids 7 to 26 (20 residues), 23.5 bits, see alignment (E = 5.7e-09) TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 7 to 32 (26 residues), 21.5 bits, see alignment (E = 1.1e-08) PF20256: MoCoBD_2" amino acids 18 to 198 (181 residues), 65.6 bits, see alignment E=8.3e-22 amino acids 593 to 672 (80 residues), 54.9 bits, see alignment E=1.5e-18 PF02738: MoCoBD_1" amino acids 317 to 551 (235 residues), 112.4 bits, see alignment E=3e-36

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 84% identity to pfo:Pfl01_2174)

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VYC0 at UniProt or InterPro

Protein Sequence (722 amino acids)

>AO353_23435 twin-arginine translocation pathway signal protein (Pseudomonas fluorescens FW300-N2E3)
MNAKIETSRRDFLKSTAVLGAGLVVAFVIPGANRFAQAASTPDAVFAPNAFLRIAPDGSV
TILLGHSEMGQGIWTGLTMLIAEELDADWTKIRVEHAPASAADYGLPAFGGMQITGGSTS
TWMEFDRYRQAGAAARLMLIEAAAKRFNVAPSKIQTEPGVVIAGDQRATYGELANDAGQL
PVPDPASIKLKDAKDWKIIGKPTKRLDTPEKITGQAKFGMDVQFDGLLTAVVARPPVFGG
SVKSFDGAAALALPGVHKVVQVPTGIAVVADHYWAAKLGRDALKIDWDLGPNAGLDSDAL
LQNFRKLAAIPGTPASQAGDISAALSKAVKTIDVEYSVPYLAHAPMEPLNCTVKITPDKC
EIWTGTQFQTLDQMIAAKITGLKPEQVEIHTEFLGGGFGRRANPTSDFVSEAVQVAKAAV
APVKTVWSREDDIRGGYYRSAFLHHARIGLDAKGMPQAWQHVLVGQSIMDGTPFEATMVK
NGVDATSVEGVADSPYIKGLANHLVDLHSPKTGISVLWMRSVGHTHTAFVVESLIDELAT
AAGKDPVEYRRTLLKEHPRHLGVLNLAVEKANWKAPLPAGHALGVAVHESFGSYVAQVAE
VSQDNLKIRVHRVVCAVDCGVVVNPASIAAQMESGITFGLGFTLHSKLTFKDGKVVQSNY
HDFQVLRLNEMPVVEVHIVPSTEKPGGIGETGVPPTAPAVANAVFALTGQRLRELPLQLA
GV