Protein Info for AO353_22940 in Pseudomonas fluorescens FW300-N2E3

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1113 TIGR02456: trehalose synthase" amino acids 16 to 561 (546 residues), 938.5 bits, see alignment E=1.1e-286 PF00128: Alpha-amylase" amino acids 39 to 397 (359 residues), 241.8 bits, see alignment E=2.4e-75 PF16657: Malt_amylase_C" amino acids 476 to 558 (83 residues), 92.4 bits, see alignment 2.2e-30 TIGR02457: putative maltokinase" amino acids 578 to 1098 (521 residues), 613.7 bits, see alignment E=3e-188 PF18085: Mak_N_cap" amino acids 610 to 695 (86 residues), 31.4 bits, see alignment 4.9e-11

Best Hits

KEGG orthology group: K05343, maltose alpha-D-glucosyltransferase [EC: 5.4.99.16] (inferred from 71% identity to avn:Avin_28000)

Predicted SEED Role

"Trehalose synthase (EC 5.4.99.16)" in subsystem Trehalose Biosynthesis (EC 5.4.99.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VYR1 at UniProt or InterPro

Protein Sequence (1113 amino acids)

>AO353_22940 alpha-amylase (Pseudomonas fluorescens FW300-N2E3)
MAKKPRSATFSNDPLWYKDAVIYQVHVKSYFDSNNDGIGDFPGLIAKLDYIAELGVNTIW
LLPFYPSPRRDDGYDIAEYRGVHRDYGTLTDAKRFIAEAHNRGLRVITELVINHTSDQHP
WFQRARKAKPGSAARDFYVWSDDDQKYDGTRIIFLDTEKSNWTWDPVAGQYFWHRFYSHQ
PDLNFDNPQVMKAVLSVMRYWLDLGIDGLRLDAIPYLIERDGTNNENLPETHAVLKQIRA
EIDAHYPDRMLLAEANQWPEDTQLYFGDVHGDNGDECHMAFHFPLMPRMYMALAQEDRFP
ITDILRQTPEIPANCQWAIFLRNHDELTLEMVTDKERDYLWNYYAADRRARINLGIRRRL
APLMERDRRRVELLNSLLLSMPGTPTLYYGDEIGMGDNIYLGDRDGVRTPMQWSIDRNGG
FSRADPASLVLPPIMDPLYGYLSVNVETQAGDPHSLLNWTRRMLAIRKQSKAFGRGSLKM
LSPSNRRILAYTREYTGDDGKHEIILCVANVSRSAQAAELDLSAFAGMVPVEMLGGNAFP
PIGQLNFLLTLAPYGFYWFVLAAENQMPSWHVEPAQSLPDFTTLVLKKRMEELLEAPSRT
ILEQSALPSWLHNRRWFAGKDSRIETVHIAYGVRFGDPLHPVLFSEIEVVNDGQTSRYQL
PFGFLAEDQVGAALPQQLALARVRRGRQVGLVTDAFSLDSFVRAVIQAMQAYTVLDASDG
EIRFEPSAGLADLNLQADCDVRYLSAEQSNSSAVIGGSVVLKLIRKVASGVHPELEMGAY
LTSAGFKHISPLLGSVIRRDRQGEDTLLMIAQGYLSNQGDAWEWTQNNLERAIRDELAEA
ISEQEQHYNALGELKDFAGMLGQRLGEMHQILAAPGDNPAFGSRVSTHKDQQALAKAIAA
QLEHALQLLKQHQARLSPEDQVLVVRLQEQKRAILRHIQELAAKAVGGLQMRVHGDLHLG
QVLVIKGDAYLIDFEGEPARPLHERRGKHSPYKDVSGVLRSFDYAAAMALDGQGVEVTAQ
TLAARQRVTERYLREATATFVQAYRQAAASLAHAWQDPAAEDAALALFCLEKAAYEVGYE
AENRPAWLPVPLHGLYGLLSGLKPFSYVSGEQS