Protein Info for AO353_17220 in Pseudomonas fluorescens FW300-N2E3

Annotation: alpha-ketoglutarate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 transmembrane" amino acids 30 to 49 (20 residues), see Phobius details amino acids 58 to 80 (23 residues), see Phobius details amino acids 92 to 112 (21 residues), see Phobius details amino acids 118 to 138 (21 residues), see Phobius details amino acids 157 to 179 (23 residues), see Phobius details amino acids 191 to 210 (20 residues), see Phobius details amino acids 239 to 259 (21 residues), see Phobius details amino acids 278 to 297 (20 residues), see Phobius details amino acids 309 to 327 (19 residues), see Phobius details amino acids 334 to 357 (24 residues), see Phobius details amino acids 368 to 390 (23 residues), see Phobius details amino acids 402 to 420 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 21 to 217 (197 residues), 97.4 bits, see alignment E=1.4e-31 amino acids 219 to 416 (198 residues), 33.2 bits, see alignment E=4.2e-12 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 26 to 415 (390 residues), 468.2 bits, see alignment E=1.2e-144 PF07690: MFS_1" amino acids 53 to 381 (329 residues), 95.9 bits, see alignment E=3.7e-31 amino acids 268 to 421 (154 residues), 37.2 bits, see alignment E=2.6e-13 PF12832: MFS_1_like" amino acids 259 to 416 (158 residues), 25.3 bits, see alignment E=1.1e-09

Best Hits

Swiss-Prot: 70% identical to KGTP_ECOLI: Alpha-ketoglutarate permease (kgtP) from Escherichia coli (strain K12)

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 90% identity to pfo:Pfl01_1272)

MetaCyc: 70% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"dicarboxylic acid transporter PcaT" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VW85 at UniProt or InterPro

Protein Sequence (431 amino acids)

>AO353_17220 alpha-ketoglutarate permease (Pseudomonas fluorescens FW300-N2E3)
MTTITSLYTAEERGKRIFAIVGASSGNLVEWFDFYVYAFCAIYFAPAFFPSDNPTVQLVN
TAGVFAAGFLMRPIGGWLFGRVADKHGRKNSMMISVLMMCAGSLLIACLPTYKDIGVWAP
LLLLVARLIQGVSVGGEYGTTATYMSEVALKGQRGFFASFQYVTLIGGQLLAVSLVVILQ
QFLTEDDLRAWGWRIPFVVGAAAALISLFLRRSLKETTSKEMRENKDAGSMAALFRNNTA
AFITVLGFTAGGSLIFYTFTTYMQKYLVNTAGMPAKTASYIMTGALFLYMCMQPLFGTLA
DKIGRRNSMLWFGALGTLFTVPILLTLKSVTNPFLAFGLITVALAIVSFYTSISGLVKAE
MFPPQVRALGVGLAYAVANAIFGGSAEYVALSLKSVGIENSFYWYVTAMMAIAFLFSLRL
PRQAAYLENEL