Protein Info for AO353_16920 in Pseudomonas fluorescens FW300-N2E3

Annotation: proline--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 566 (566 residues), 811.6 bits, E=2.2e-248
• PF00587: tRNA-synt_2b
  • amino acids 95 to 457 (363 residues), 118.9 bits, E=4.2e-38
• PF04073: tRNA_edit
  • amino acids 257 to 375 (119 residues), 91.8 bits, E=5.2e-30
• PF03129: HGTP_anticodon
  • amino acids 473 to 567 (95 residues), 58 bits, E=1.4e-19

Best Hits

Swiss-Prot: 96% identical to SYP_PSEF5: Proline--tRNA ligase (proS) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 96% identity to pba:PSEBR_a1142)

MetaCyc: 67% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• anguibactin biosynthesis (2/8 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Sequence Analysis Tools

See A0A0N9WJI2 at UniProt or InterPro

Protein Sequence (571 amino acids)

>AO353_16920 proline--tRNA ligase (Pseudomonas fluorescens FW300-N2E3)
MRTSQYLLATQKETPSDAVVVSHQLMLRAGMIRKLASGLYTWLPMGLRVMRKVEAVVREE
MNAAGSLEVLMPSTQPAELWQESGRWEEYGPELLRFKDRHGRDFCAGPTHEEVITDLMRN
ELSSYKQLPINLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFHADQASLQITYDRMHQA
YCNVFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSNGSDYAANIEKAEAVP
RETSRAAPSEELRLVDTPNAKTIAQLVEGFNLPIEKTIKTLVVHAEEKGKLIALIIRGDH
ELNEIKAANQPGVASPLVMASEAELRDAIGAGAGSLGPLNLPLPIIIDRSVELMSDFGIG
ANIDDKHYFGVNWERDLPVPTVADLRNVVSGDPSPDGKGTLEIKRGIEVGHIFQLGNKYS
KAMKCEVLGENGKPVTLEMGCYGIGVSRVVAAAIEQNNDEKGIIWSDALAPFQIALVPLR
YETEQVREATDKLYAELTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAE
GNLEYKSRTEAEPQALPVADVLSFLQARIRR