Protein Info for AO353_15985 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): Alpha,alpha-phosphotrehalase (EC 3.2.1.93)
Rationale: Specifically important for utilizing D-Trehalose dihydrate. Automated validation from mutant phenotype: the predicted function (3.2.1.93) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: trehalose-6-phosphate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 549 TIGR02403: alpha,alpha-phosphotrehalase" amino acids 4 to 545 (542 residues), 864.6 bits, see alignment E=1.5e-264 PF00128: Alpha-amylase" amino acids 26 to 363 (338 residues), 408.2 bits, see alignment E=5.4e-126 PF11941: DUF3459" amino acids 450 to 510 (61 residues), 27 bits, see alignment E=7.6e-10 PF16657: Malt_amylase_C" amino acids 467 to 542 (76 residues), 35 bits, see alignment E=1.9e-12

Best Hits

Swiss-Prot: 59% identical to TREC_BACSU: Trehalose-6-phosphate hydrolase (treA) from Bacillus subtilis (strain 168)

KEGG orthology group: K01226, trehalose-6-phosphate hydrolase [EC: 3.2.1.93] (inferred from 88% identity to pfs:PFLU5039)

MetaCyc: 59% identical to trehalose-6-phosphate hydrolase (Bacillus subtilis)
Alpha,alpha-phosphotrehalase. [EC: 3.2.1.93]

Predicted SEED Role

"Trehalose-6-phosphate hydrolase (EC 3.2.1.93)" in subsystem Trehalose Uptake and Utilization (EC 3.2.1.93)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.93

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H093 at UniProt or InterPro

Protein Sequence (549 amino acids)

>AO353_15985 Alpha,alpha-phosphotrehalase (EC 3.2.1.93) (Pseudomonas fluorescens FW300-N2E3)
MQDWQRSVIYQIYPKSFHSHAGNPTGDLLGVVAKLDYLQWLGVDCLWITPFLRSPQRDNG
YDISDYYAIDPSYGTMADCELLIAEAGKRGIKLMLDIVVNHTSIEHSWFQQARSSLDNPY
RDFYIWRDQPNNWESKFGGSAWEYEAQTGQYFLHLFDHTQADLNWDNPQVRAEVFKLMRF
WRDKGVGGFRLDVINLISKPADFPEDHTDGRRFYTDGPNVHEYLQEMHREVFEGHDLINV
GEMSSTSLEHCIRYSRPDSKELSMTFNFHHLKVDYPNMQKWVRADFDFLELKRILSDWQT
GMQAGGGWNALFWCNHDQPRVVSRFGDDGEHRVVSAKMLGTALHFLQGTPFIYQGEELGM
TNPGFERIEQYRDVETLNIYRLKREAGESEASSMAAIMQKSRDNSRTPMQWSALPNAGFS
SSEPWIGVPANAMQINVENQLDDTTSVLHHYRQLIALRRSEPLIQDGVYRQLLPTHKQVW
VYLREGEGERLLVVNNFYGTACEVELPERVITDCMLQRLVISNYPDSEVRKQQLFLRPYE
SFVLHLTDH