Protein Info for AO353_11865 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 602 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 281 to 300 (20 residues), see Phobius details PF02743: dCache_1" amino acids 43 to 267 (225 residues), 54.9 bits, see alignment E=1.4e-18 PF00512: HisKA" amino acids 369 to 434 (66 residues), 40.2 bits, see alignment E=4.4e-14 PF02518: HATPase_c" amino acids 480 to 587 (108 residues), 73.5 bits, see alignment E=2.9e-24

Best Hits

Swiss-Prot: 67% identical to DCTB_PSEAE: C4-dicarboxylate transport sensor protein DctB (dctB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 92% identity to pfo:Pfl01_0288)

Predicted SEED Role

"Signal transduction histidine kinase regulating C4-dicarboxylate transport system"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZZ8 at UniProt or InterPro

Protein Sequence (602 amino acids)

>AO353_11865 ATPase (Pseudomonas fluorescens FW300-N2E3)
MTPTLPRRPRWRSLALLALCLAPLLWPLQHLAERYYRSELVGQNRQTLDLYVANLLGTLH
RYEVLPQILGDLPALRAVLAAPYEPQATDNANRLLKDIRAQTGAEVMYLMDTHGKTLAAS
NWDKHDSFVGRNFAFRPYFSEAMQGRLGRFFGLGTTSAKRGYFFAGAVRDGEQIIGVLVV
KVDLDHTESLWGKTPEQLLLTDHNGVVIITSRPEWRFRATRPLTDEERHAISAVQPYPTR
DPQPLNLNPDAWLIQTQQIEETGWSVSILAPRTLIDRPVRTVVAIGGATLLVLMLLLGLM
MQRRRHYLQRIAFEAKTRRELEARVAERTSDLEGLNRRLKQEVLEREQAQQELVRAQDDL
VQAGKLSALGTMSASISHELNQPLAAIRSYAENAEVLLDHQRTDDARGNLKLISELTGRM
ASIIAHLRAFARRDRHAPESVALQPALDDALALLAKRRRAMEVELIRDLPAATLWVEAGE
TRLRQVLGNLLANALDALTEKGPPRKLWLSAESTADGVNLYIRDNGPGFCMEALGRAGEP
FYTTKTRTQGLGLGLAICDTLMRAFGGELSFANHKEGGALITLRLRAGAPGVSLQPSEDR
SE