Protein Info for AO353_10975 in Pseudomonas fluorescens FW300-N2E3

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details transmembrane" amino acids 169 to 191 (23 residues), see Phobius details PF16767: KinB_sensor" amino acids 40 to 160 (121 residues), 91.9 bits, see alignment E=1.8e-29 PF00672: HAMP" amino acids 194 to 245 (52 residues), 42 bits, see alignment 3.7e-14 TIGR00229: PAS domain S-box protein" amino acids 258 to 374 (117 residues), 38.1 bits, see alignment E=7.4e-14 PF13188: PAS_8" amino acids 259 to 313 (55 residues), 28.5 bits, see alignment 4.3e-10 PF00989: PAS" amino acids 260 to 367 (108 residues), 38.9 bits, see alignment E=3.2e-13 PF08448: PAS_4" amino acids 265 to 372 (108 residues), 46.8 bits, see alignment E=1.2e-15 PF00512: HisKA" amino acids 377 to 444 (68 residues), 52.8 bits, see alignment E=1.3e-17 PF02518: HATPase_c" amino acids 491 to 596 (106 residues), 101.3 bits, see alignment E=1.7e-32

Best Hits

KEGG orthology group: K11383, two-component system, NtrC family, sensor histidine kinase KinB [EC: 2.7.13.3] (inferred from 82% identity to pfo:Pfl01_0047)

Predicted SEED Role

"Sensory box histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZX7 at UniProt or InterPro

Protein Sequence (596 amino acids)

>AO353_10975 histidine kinase (Pseudomonas fluorescens FW300-N2E3)
MKLAMKLRTRLFLSISALITVALLGLVLGLVSVMQIASNQEALIRDNFITLDLELKLRQT
LDDQLINMLSIKPDLEALESSERRYFELLDQGIEHENSFNGEHHGFEQARTDYLSFLKAF
SLTQEPSGKLAGNRDLTEKFNVLRNGLIAEHKQALDNIDETQRNAREHALLIAGLLGLVG
LAVLAIGFITAHGIARRFGEPIEALAKAADNIGQGNFDVTLPISPAVELTQLTRRFGIMA
EALREHQATNVDELLAGQQRLQAVLDSIDDGLLMIDRQGQLEHLNPVAQRQLGWDEGRLG
QGLGTALQRPELDEQLQTVLRGGTLERAPEDLSIEVDGESRLLTYSLTPVIHPQGHILGA
VMVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLFRERAHFTEDSREADLLNT
VNEEMQRLMQLINDLLNFSRYQNGLQKLTLAPCNIDDLLEQAQERFAERANEQSIELLVE
VHTPLPRLQADQAQLDRVLDNLIDNALRHTSHHGQIRLQARRHGERVIVSVEDNGEGIAY
GQQGRIFEPFVQVGRKKGGAGLGLALCKEIVQLHGGRMGVYSRPGQGTQFYMALPL