Protein Info for AO353_06770 in Pseudomonas fluorescens FW300-N2E3

Annotation: NAD-dependent dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 6 to 233 (228 residues), 47.7 bits, E=5.4e-16
• PF02719: Polysacc_synt_2
  • amino acids 6 to 260 (255 residues), 55 bits, E=3.3e-18
• PF05368: NmrA
  • amino acids 6 to 121 (116 residues), 30.4 bits, E=1.2e-10
• PF01370: Epimerase
  • amino acids 6 to 243 (238 residues), 229.8 bits, E=1.5e-71
• PF01073: 3Beta_HSD
  • amino acids 7 to 230 (224 residues), 83.7 bits, E=5.3e-27
• PF16363: GDP_Man_Dehyd
  • amino acids 7 to 255 (249 residues), 177.6 bits, E=2.1e-55
• PF07993: NAD_binding_4
  • amino acids 8 to 193 (186 residues), 39.9 bits, E=1.2e-13
• PF13460: NAD_binding_10
  • amino acids 10 to 157 (148 residues), 62.7 bits, E=1.9e-20

Best Hits

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 91% identity to pba:PSEBR_a5051)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (9/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose detoxification (1/3 steps found)
• D-galactose degradation I (Leloir pathway) (2/5 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Sequence Analysis Tools

See A0A0N9WSC5 at UniProt or InterPro

Protein Sequence (309 amino acids)

>AO353_06770 NAD-dependent dehydratase (Pseudomonas fluorescens FW300-N2E3)
MADGPVLITGGAGFIGSHLADALLAKGHSVRILDDLSTGKRSNLPLDNPRVELIEGDVAD
AALVAKVMLGCSAVAHLAAVASVQASVDDPVRTHQSNFIGTLNVCEAMRQAGVKRVLFAS
SAAVYGNNGEGESIDEETAKAPLTPYAADKLAGEYYLDFYRRQHGLEPAIFRFFNIFGPR
QDPSSPYSGVISIFSERAQKGLPITVFGDGEQTRDFVYVEDLVDLLVQAIEMPTLEVGAV
NVGWNQATTLKQMLQALEEVVGQLPPIAYGPARSGDIRHSRANNQRLLQRFTFPEQTPMS
VGLARLLGR