Protein Info for AO353_04360 in Pseudomonas fluorescens FW300-N2E3

Annotation: protein-disulfide isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 243 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF10411: DsbC_N" amino acids 22 to 91 (70 residues), 75.5 bits, see alignment E=4.1e-25 PF13098: Thioredoxin_2" amino acids 116 to 238 (123 residues), 77.6 bits, see alignment E=1.8e-25

Best Hits

KEGG orthology group: K03981, thiol:disulfide interchange protein DsbC [EC: 5.3.4.1] (inferred from 94% identity to pfs:PFLU5007)

Predicted SEED Role

"Thiol:disulfide interchange protein DsbC" in subsystem Periplasmic disulfide interchange

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W837 at UniProt or InterPro

Protein Sequence (243 amino acids)

>AO353_04360 protein-disulfide isomerase (Pseudomonas fluorescens FW300-N2E3)
MRLTQIFAAAAIALVSTFAIADDAADKAIRKSLETLQLDTPIESISASPLAGLYEVKLKG
SRVLYASADGQYIVQGYVFQLKDGKPVNLTEKAERLGISKLINGIPVAETVVYPAIGETK
THITVFTDTTCPYCHKLHAEVPALNKMGIEVRYVAFPRQGLGSPGDEQLQAVWCSADKKA
AMDKMVDGKEIKAAKCANPVSKQFALGQSIGVNGTPAIVLADGQVIPGYQPAPQVAKLAL
SAK