Protein Info for ABZR87_RS13455 in Ralstonia sp. UNC404CL21Col
Annotation: dihydrolipoyl dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to DLDH_VIBCH: Dihydrolipoyl dehydrogenase (lpd) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 99% identity to rpf:Rpic12D_1616)MetaCyc: 70% identical to dihydrolipoamide dehydrogenase (Advenella mimigardefordensis DPN7)
Dihydrolipoyl dehydrogenase. [EC: 1.8.1.4]; 1.8.1.- [EC: 1.8.1.4]
Predicted SEED Role
"Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or TCA Cycle (EC 1.8.1.4)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
- 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
- glycine biosynthesis II (3/3 steps found)
- glycine cleavage (3/3 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
- 3,3'-disulfanediyldipropannoate degradation (2/4 steps found)
- 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (1/3 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glycine, serine and threonine metabolism
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.8.1.4
Use Curated BLAST to search for 1.8.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (593 amino acids)
>ABZR87_RS13455 dihydrolipoyl dehydrogenase (Ralstonia sp. UNC404CL21Col) MSVVEIKVPDIGDYKDVDVIEVMVKAGDAVTVDQALITLETDKATMDVPSDVAGKIVEVK IKVGDKVSQGTVIATAEAGAAAAPAPAQAPAPAATPAAAPAASAPAPQAAKHAGGADIEC EMLVLGSGPGGYSAAFRSADLGMNTVLVERFSTLGGVCLNVGCIPSKALLHTAAVMDEVK AMAAHGIVYSEPTVDINQLRKHKESVIGKLTGGLAGMAKARKVQVVRGVGAFLDPNHLEV QLTDGDGKATTGEKKVIRFAKAIIAAGSEAVKLPFIPEDPRIVDSTGALELREVPAKMLV IGGGIIGLEMATVYSTLGARIDVVEMLDGLMQGADRDLVKVWDKMNKSRFDKVMLKTKTV GVEAKPDGIYVKFEGEAAPAEPQRYDMVLVAVGRAPNGKRIGAEKAGVAVTDRGFIDVDK QQRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDAKQIPSVAYTDPEVAW AGLTEEQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAG DLIGEVCLAIEMGADAVDIGKTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR