Protein Info for ABZR87_RS12625 in Ralstonia sp. UNC404CL21Col

Annotation: 3-(methylthio)propionyl-CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 20 to 400 (381 residues), 258.2 bits, E=1.2e-80
• PF13193: AMP-binding_C
  • amino acids 448 to 522 (75 residues), 53.4 bits, E=4e-18

Best Hits

Swiss-Prot: 55% identical to DMDB_RUEPO: 3-methylmercaptopropionyl-CoA ligase (dmdB) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 99% identity to rpf:Rpic12D_1496)

MetaCyc: 55% identical to 3-methylmercaptopropionyl-CoA ligase (Ruegeria pomeroyi DSS-3)
RXN-12571 [EC: 6.2.1.44]

Predicted SEED Role

"small molecule metabolism; fatty acid biosynthesis"

MetaCyc Pathways

• methylthiopropanoate degradation I (cleavage) (2/6 steps found)
• superpathway of dimethylsulfoniopropanoate degradation (2/8 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis II
• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Caprolactam degradation
• Ethylbenzene degradation
• Geraniol degradation
• Limonene and pinene degradation
• Propanoate metabolism
• Tyrosine metabolism
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.-

Use Curated BLAST to search for 6.2.1.- or 6.2.1.44

Protein Sequence (542 amino acids)

>ABZR87_RS12625 3-(methylthio)propionyl-CoA ligase (Ralstonia sp. UNC404CL21Col)
MALMGQMMSAPLLISTIIEHAARNSGSTEVVSRRVEGDIHRTTYRQVRDRSKQLANALAA
LGVQPGERVGTLAWNGYRHLEIYYGVSGSGSVCHTINPRLFPDQIAYIVNHADDQYVFFD
LTFVPLVEGIAPHCPNVKGWVAMTDRAHMPASSVPMLCYEELLDAQSADYTWPQFDENTA
SSLCYTSGTTGNPKGALYSHRSTVLHSYASAMPDALGCSAQDVILPVVPMFHVNAWGLPY
SVPLVGAKLVFPGPKLDGASLFELFEQEKVTFSAGVPTVWLGLLQHVQANKLKFSTFRRT
VIGGSAAPPAMIRTLNELDVEVIHAWGMTEMSPLGTTCKLMGKHADLPDEAKQHVLERQG
RAIYGVEMKIVDAEGHELPWDGKAFGDLYVRGPWTIQSYYRNEVSPLVDGWFPTGDVANI
DADGYMQITDRSKDVIKSGGEWISSIDVENVAAAHPGVHMAACISCHHPKWDERPLLVVM
KKPGVELTREEMLKFFEGKVAKWWIPDDVVFVTEIPLTATGKMQKLKLREQFKDYQFPAV
QA