Protein Info for ABZR87_RS09435 in Ralstonia sp. UNC404CL21Col

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 TIGR01063: DNA gyrase, A subunit" amino acids 10 to 857 (848 residues), 1206.5 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 513 (482 residues), 539.9 bits, see alignment E=4.8e-166 PF03989: DNA_gyraseA_C" amino acids 545 to 592 (48 residues), 40.7 bits, see alignment 1.3e-14 amino acids 595 to 644 (50 residues), 35.4 bits, see alignment 5.6e-13 amino acids 652 to 695 (44 residues), 37.7 bits, see alignment 1.1e-13 amino acids 700 to 745 (46 residues), 51.6 bits, see alignment 5.1e-18 amino acids 760 to 806 (47 residues), 45.9 bits, see alignment 3e-16 amino acids 810 to 856 (47 residues), 43.9 bits, see alignment 1.2e-15

Best Hits

Swiss-Prot: 61% identical to GYRA_SALTY: DNA gyrase subunit A (gyrA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 83% identity to bcj:BCAL2957)

MetaCyc: 60% identical to DNA gyrase subunit A (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (889 amino acids)

>ABZR87_RS09435 DNA gyrase subunit A (Ralstonia sp. UNC404CL21Col)
MDQFAKETLPVSLEEEMRSSYLAYAMSVIVGRALPDVRDGLKPVHRRALYSMHELNNDWN
RPYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQNFSLRYMLVDGQGNFGSVDGDNAAAM
RYTEIRLSKIAHELLADLDKETVNFEPNYDGSETEPSVLPARIPNLLINGSSGIAVGMAT
NIPPHNLNEVVDGCLHLLRNPEATVDELIELIPAPDFPTAGIIYGISGVREGYRTGRGRV
VMRAKTHFEDIDRGQRQAIIVDELPYQVNKRTLLERIAELVTEKRIEGISDIRDESDKSG
MRVVIELKRGEVPEVVLNNLYKNTQLQDTFGMNMVALVDGQPRLLNLRQMLECFLLHRRE
VVTRRTVFDLRKARERGHILEGLAVALANIDEFIAIIKAAPTPPVAKAELMSRSWDSGLV
RDMLVRAESETSGGGAAYRPEGLLPAFGMQADGLYKLSDTQAQEILQMRLQRLTGLEQDK
IVQEYREVMAQIADLLDILARPERITAIIVEELTAIRAEFGDERRSQIEHNATELDTEDL
ITPQDLVVTLSHSGYMKSQPISEYRAQKRGGRGKQAAATKEDDWIDTLFVANTHDYILCF
SNRGRLYWLKVWEVPQGSRNSRGRPIVNMFPLSPGEKINVILPVKQFDEQHFVFMATSKG
TVKKTALTEFSNPRKAGIIAVDLDEGDFLIGADITDGQHDVMLFSDAGKAVRFDENDVRP
MGRQARGVRGMNLEENQQVIAMLVAPAETAGEGAQSGAGSVLTATENGYGKRTPISEYTR
HGRGTKGMIAIQTSERNGKVVAAALVAPEDEIMLITTGGVLIRTRVDEIREMGRATQGVT
LISVGEGNKLSGLQRVMESDAEGADAVEGADAADAAPGADAQSDDSSET