Protein Info for ABZR87_RS06540 in Ralstonia sp. UNC404CL21Col

Annotation: phosphocholine-specific phospholipase C

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR03396: phospholipase C, phosphocholine-specific" amino acids 5 to 698 (694 residues), 866.5 bits, see alignment E=7.3e-265 PF04185: Phosphoesterase" amino acids 48 to 432 (385 residues), 373.2 bits, see alignment E=1.5e-115 PF05506: PLipase_C_C" amino acids 505 to 591 (87 residues), 90.8 bits, see alignment E=9.2e-30 amino acids 606 to 685 (80 residues), 54.7 bits, see alignment E=1.6e-18

Best Hits

Swiss-Prot: 70% identical to PHLN_BURPS: Non-hemolytic phospholipase C (plcN) from Burkholderia pseudomallei (strain K96243)

KEGG orthology group: K01114, phospholipase C [EC: 3.1.4.3] (inferred from 97% identity to rpi:Rpic_0175)

Predicted SEED Role

"Phospholipase C"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.3

Use Curated BLAST to search for 3.1.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (700 amino acids)

>ABZR87_RS06540 phosphocholine-specific phospholipase C (Ralstonia sp. UNC404CL21Col)
MTSNSRRGFLRSAAQLAAASAAMGAVPEGIRQALAIPAHNAHRTIEDVQHIVILMQENRS
FDHYFGTLRGVRGYGDTRTVTLPTGKSVWHQPMAGGVGEVLPFRPSAPDLGLQFLQDLPH
GWNDTHLAVNGGRYDGWVPHKGTTTMAYLTRQDIPFHYALADAFTICDAYHCATPTSTDP
NRYYMWTGYVGNDNVGGGPVIDNAEAGYGWSTYPEVLEAAGISWKIYQDIGTGLDAAGSW
GWTSDAYIGNYGDNSLLYFKQYQNAQPGSPLYDKARTGTNAAAGDGYFDILKRDVQYGTL
PQVSWIAAPEAFSEHPNWPANYGAWYIDQVLQILTSNPEVWSKTAVFITYDENDGFFDHL
VPPFASSGSNGLSTVDTSGEYFPGNSTYGAGSYGLGQRVPMLVVSPWSKGGFVCSETFDH
TSIIRFIEQRFKRTHNVRSPNISKWRRAVCGDLTSAFNFATPNEVVPALPSTTAYVPKDK
LRHASYVPLPPLVQAVPKQEAGGRPSRALPYELFVRGRQDEAAGKFQLEFVNTGDLGAAF
LVYTTGSTDAPRTYTVEAGKRLSDKLAVNAGGAYDFTVHGPNGFLRRFVGKLTLSGLLRP
HGRALEVKEGYDVANGNLQLRLINHGRAPTVFTIKSAYTGESVQREVRGGDDAAVYLDLR
SQHAWYDMTVTASSDAGFERRLAGHVETGRVSASDPALSA