Protein Info for ABZR87_RS05960 in Ralstonia sp. UNC404CL21Col

Annotation: thiamine pyrophosphate-requiring protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 598 PF02776: TPP_enzyme_N" amino acids 4 to 121 (118 residues), 121.5 bits, see alignment E=2.5e-39 PF00205: TPP_enzyme_M" amino acids 198 to 328 (131 residues), 96.3 bits, see alignment E=1.9e-31 PF02775: TPP_enzyme_C" amino acids 388 to 543 (156 residues), 96.7 bits, see alignment E=1.8e-31

Best Hits

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 97% identity to rpi:Rpic_0068)

Predicted SEED Role

"Pyruvate oxidase (EC 1.2.3.3)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.3.3 or 1.2.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (598 amino acids)

>ABZR87_RS05960 thiamine pyrophosphate-requiring protein (Ralstonia sp. UNC404CL21Col)
MRATVSDFLVARLYDWGVRRIFGYPGDGINGMFGALQRAGGKIEFVQVRHEEMAAFMASA
HAKFTGELGVCMATSGPGATHLITGLYDARLDHMPVLAITGQQARTAVGGHYQQEVDLAA
MFRDVAGAFVHQASMPAQVRHLLDRAVRIAIGQRRVTALVLPNDLQEMPYTEPPRAHGTV
HSGVGYSAPSVIPQAADLERAADILNSGRRVAMLVGAGALGASAEVAAVAETLGAGVAKA
LLGKAVLPDELPYVTGAIGLLGTEPSWELMSHCDTLLMVGSGFPYAEFLPKEGQARGVQI
DLDPGMLGLRYPMEVNLVGDSAQTLRALLPLLQRKTDRYWPDRIARWNESWWKTLEDRAL
QPAYPVNPQRVFWELSPKLPSNVIVTSDSGTCANWYARDLKFKPGMMGSLSGGLASMGAA
VPYAIAAKFAHPDRPVIALVGDGAMQMNNMAELITVSKYWKRWKSPKWICMVLNNQDLNE
VTWEQRVMEGDPKFPASQDIPDVPYHRFAELIGLKGIYVDNPDQLAGAWDEALDADRPVV
LEVKTDPDVPPLPPHVTLQQARNFATALAYGDPDASGVLRGTARQVLSALFPVHKDKE