Protein Info for ABZR87_RS01295 in Ralstonia sp. UNC404CL21Col

Annotation: protein translocase subunit SecF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 141 to 159 (19 residues), see Phobius details amino acids 166 to 187 (22 residues), see Phobius details amino acids 193 to 215 (23 residues), see Phobius details amino acids 249 to 297 (49 residues), see Phobius details PF07549: Sec_GG" amino acids 32 to 59 (28 residues), 32.6 bits, see alignment (E = 4.1e-12) TIGR00966: protein-export membrane protein SecF" amino acids 38 to 288 (251 residues), 243.5 bits, see alignment E=2.8e-76 TIGR00916: protein-export membrane protein, SecD/SecF family" amino acids 105 to 285 (181 residues), 164.8 bits, see alignment E=2.5e-52 PF02355: SecD_SecF" amino acids 112 to 296 (185 residues), 215.5 bits, see alignment E=4.4e-68

Best Hits

Swiss-Prot: 42% identical to SECF_PSEAE: Protein translocase subunit SecF (secF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03074, preprotein translocase subunit SecF (inferred from 98% identity to rpf:Rpic12D_2550)

MetaCyc: 38% identical to Sec translocon accessory complex subunit SecF (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Protein-export membrane protein SecF (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>ABZR87_RS01295 protein translocase subunit SecF (Ralstonia sp. UNC404CL21Col)
MEFFRIRRDIPFMKHALILNALSFLTFIAAVFFLFHKGLHLSVEFTGGTVMEVAYTQAAD
LPKIRGDVEKLGYADAQVQNFGTSRDVMIRLPLKTGPDGKPIASAVQSEQVMTALNAANP
DAKLQRVEFVGPQVGKELATDGLLALLFVVVGIVIYLSIRFEWKFAVAGVIANLHDVVII
LGFFAFFQWEFSLSVLAGVLAVLGYSVNESVVIFDRIREAFRKYRKMNTHEVIDHAITST
ISRTIITHGSTEMMVLSMLVFGGPTLHYFALALTIGILFGIYSSVFVAAALCMWLGVKRE
DLVKADKKAGGPKEDDPNFGAQV