Protein Info for ABZR86_RS13485 in Dyella japonica UNC79MFTsu3.2

Annotation: malate synthase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF20656: MS_N
  • amino acids 17 to 69 (53 residues), 58.3 bits, 6.3e-20
• TIGR01344: malate synthase A
  • amino acids 26 to 542 (517 residues), 720.3 bits, E=4.7e-221
• PF01274: MS_TIM-barrel
  • amino acids 164 to 408 (245 residues), 355.4 bits, E=1.8e-110
• PF20659: MS_C
  • amino acids 415 to 542 (128 residues), 95.9 bits, E=3.4e-31

Best Hits

Swiss-Prot: 60% identical to MASY_MYXXD: Malate synthase (mls) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 66% identity to psu:Psesu_0138)

MetaCyc: 57% identical to malate synthase A (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• L-arabinose degradation IV (5/8 steps found)
• chitin deacetylation (2/4 steps found)
• superpathway of glycol metabolism and degradation (4/7 steps found)
• D-xylose degradation IV (2/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)
• superpathway of pentose and pentitol degradation (12/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See A0A1I2EMS2 at UniProt or InterPro

Protein Sequence (543 amino acids)

>ABZR86_RS13485 malate synthase A (Dyella japonica UNC79MFTsu3.2)
MAVPQERLPQSSIRIEGETGPGHESILTPAALAFLADLHHRFNDRRLDLLAARRTRQARY
DAGELPDFRADTLAIREGRWTVGKIPAALQDRRVEITGPVERKMIINALNSGAKVFMADF
EDSSAPTLANQFDGQINLRDAVNGTIGYTSPEGKTYRVGHDPAVLVVRPRGWHLPERHLT
VDGEPMSGALVDFGLYAFHNARALQARDRGPYFYLPKLEAMEEAALWHEAMAAAEEALGL
PAHCMKATVLIETLPAVFQMHEILHALRERAVGLNCGRWDYIFSYLKTFRGHRDRLLPER
GQVLMTVPFLKAYSELLIQTCHRRGAFAMGGMAAQIPIKGDEAANEAALAKVRADKLREV
QAGHDGTWVAHPALVPVAREIFDQYMPSPNQLNVARADVQASRDQLIAPCAGTISRAGFD
NNVEVALRYTAAWLDGLGCVPIHHLMEDAATAEIARAQLWQWLHHPGNPAGGTPAGDGLE
FTDHAPIDFALFDQALAAHTHRLRASTHPGAARADDAAALLATLTHAEQLGDFLTLPAYD
RLA