Protein Info for ABZR86_RS10525 in Dyella japonica UNC79MFTsu3.2

Annotation: proline--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 561 (561 residues), 764.6 bits, E=4e-234
• PF00587: tRNA-synt_2b
  • amino acids 95 to 456 (362 residues), 116.6 bits, E=2e-37
• PF04073: tRNA_edit
  • amino acids 257 to 374 (118 residues), 102.4 bits, E=2.8e-33
• PF03129: HGTP_anticodon
  • amino acids 474 to 562 (89 residues), 55 bits, E=1.1e-18

Best Hits

Swiss-Prot: 66% identical to SYP_XANCB: Proline--tRNA ligase (proS) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 66% identity to xca:xccb100_3716)

MetaCyc: 59% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• anguibactin biosynthesis (1/8 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Sequence Analysis Tools

See A0A1I2FJ57 at UniProt or InterPro

Protein Sequence (565 amino acids)

>ABZR86_RS10525 proline--tRNA ligase (Dyella japonica UNC79MFTsu3.2)
MRLSQYHLATVKEVPADAEIASHQLMLRTGMIRKLAAGLYTWAPLGLRVMRKVEQVVREE
MDRAGAIEMLMPTIQPRELWEETTRWQKFGGQLLKIKDRKEQEFCYAPTAEEVVTDFARN
ELKSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFVMKDAYSFHLGQDSLGQTYQAMYDA
YTRIFSRLGLKFRAVQADTGAIGGNASHEFQVLADAGEDALVFSDGSDYAANIEKAEALA
PTTSRPAPAAELERVDTPTQKTIEDVAAFLKVEPSQCVKTILVQGSKGLVALCVRGDHEV
NEVKAGKLAEMPGESALATEAEILAVTGTRPGFIGPVGLPAEIPVIVDRSAAVLADFICG
GNQDGTHYSGANWERDARVTRVEDIRKVVEGDPSPDGKGSLRMGRGIEVGHVFQLGGKYA
EALKATVLDESGKAQVMLMGCYGIGVSRIVAAAIEQCHDEAGILWPEAMAPWRVAVCMIN
PKGLPEVTQAAETLYAQLQERGIEVVLDDRGLRPGAMFADMELIGIPHRVVVSERGVAAG
TLEYRARNAAESRNIDSAELFALLG