Protein Info for ABZR86_RS10370 in Dyella japonica UNC79MFTsu3.2

Annotation: glutathione synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 signal peptide" amino acids 1 to 15 (15 residues), see Phobius details TIGR01380: glutathione synthase" amino acids 3 to 317 (315 residues), 437.8 bits, see alignment E=9.5e-136 PF02951: GSH-S_N" amino acids 3 to 121 (119 residues), 151 bits, see alignment E=2.2e-48 PF02955: GSH-S_ATP" amino acids 125 to 303 (179 residues), 238 bits, see alignment E=6.7e-75 PF08443: RimK" amino acids 139 to 315 (177 residues), 48.4 bits, see alignment E=1.3e-16

Best Hits

Swiss-Prot: 68% identical to GSHB_XANAC: Glutathione synthetase (gshB) from Xanthomonas axonopodis pv. citri (strain 306)

KEGG orthology group: K01920, glutathione synthase [EC: 6.3.2.3] (inferred from 69% identity to xcv:XCV3234)

MetaCyc: 54% identical to glutathione synthetase (Escherichia coli K-12 substr. MG1655)
Glutathione synthase. [EC: 6.3.2.3]

Predicted SEED Role

"Glutathione synthetase (EC 6.3.2.3)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Heat shock dnaK gene cluster extended (EC 6.3.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I2FL20 at UniProt or InterPro

Protein Sequence (325 amino acids)

>ABZR86_RS10370 glutathione synthase (Dyella japonica UNC79MFTsu3.2)
MALSVAVLMDPIGAIKIAKDTTFAMLLEAQRRGHALLYMEHGDLAVRDGRAWARLRPLSV
KDDPAGWFSLGEARWQELAEVDVVLLRKDPPVDSQFIYDTMVLSLAQRAGVTVVNDPQAL
RDCNEKMYALDFGQCMAPTLLSRDKADLRRFVAEHGEVVLKPLDGMGGRGIFRVRAGDSN
LNSMLETLLSGGLLSGQAQFVMAQKFIPQISAGDKRILVVDGEAVPYALARIPQGDEFRG
NLAAGGRGEGVPLTERDRWIVAQVAPELRRRGLWFVGLDVIGDYLTEINVTSPTCARELD
KQFGLNICGQLFDVIEHHIHGKRSA