Protein Info for ABZR86_RS03855 in Dyella japonica UNC79MFTsu3.2

Updated annotation (from data): Nitrite reductase (NAD(P)H) (EC 1.7.1.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.1.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: nitrite reductase large subunit NirB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 860 PF07992: Pyr_redox_2" amino acids 4 to 285 (282 residues), 168.7 bits, see alignment E=6.2e-53 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 5 to 810 (806 residues), 1099.9 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 146 to 214 (69 residues), 49.9 bits, see alignment E=1.1e-16 PF18267: Rubredoxin_C" amino acids 330 to 399 (70 residues), 40.1 bits, see alignment E=8.9e-14 PF04324: Fer2_BFD" amino acids 434 to 481 (48 residues), 45.1 bits, see alignment 3.1e-15 PF03460: NIR_SIR_ferr" amino acids 570 to 632 (63 residues), 45.8 bits, see alignment 1.2e-15 PF01077: NIR_SIR" amino acids 642 to 760 (119 residues), 76 bits, see alignment E=7e-25

Best Hits

Swiss-Prot: 60% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 73% identity to bam:Bamb_3936)

MetaCyc: 60% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1I1Z2Z2 at UniProt or InterPro

Protein Sequence (860 amino acids)

>ABZR86_RS03855 Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (Dyella japonica UNC79MFTsu3.2)
MTTRLVVIGNGMVGHKLLEELAALGTPGLEITVLCEESRPAYDRVHLSEYFSGRSADELS
LVTPGFFDQPGLHLRLATRAARIDRAFKRVTTADGEVIPYDKLVFATGSSPFVPPIPGND
RPGCLTYRTLDDLAALAGWGAQSKRGVVVGGGLLGLECAKAVRDMGLETHVVEFAPRLMA
VQVDDGGGRILHRTIEELGLVVHTGKNTQAIVDGSGIDGANARHAMKFADGSTLETDLIV
FSAGIRPRDELARQCDLGVGQRGGIMVDNFCRTRDRDVYAIGECAQWGGKVFGLVAPGYD
MARVVAKHLHALLTREEARDLPEFAGADMSTKLKLMGVDVASLGDAHGATPGSRSYQFSD
ERRQVYKRLVVSEDGKRLLGGVLVGDATEYGTLLQMMLNGMDLPEAPESLILPASDGKSR
PGLAVDALPAAAQVCSCNNVSKGQLCVAVAEGATSIGALKKATRAGTTCGGCVPLVTQIM
KAEMARQGLAVNNHLCEHFAHSRQELYHLVRVERIRRFEDLLARHGQGLGCDICKPVAAS
ILASCWNEFVLQQEHASLQDSNDYFLANIQKDGTYSVVPRMPGGEVTPDGLIAVGRIAKS
YGLYTKLTGGQRVDLFGARLEQLPLIWEELIAAGFESGHAYGKSVRTVKSCVGSTWCRYG
VQDSVGTAIDLENRYKGLRSPHKLKFAVSGCARECAEAQSKDVGVIATEQGWNLYVCGNG
GMKPRHAELLAGDLDTATLIRYIDRFLMFYIRTADRLQRTSVWRDGLEGGMDYLRDVIVH
DKLGLADELEAEMALVVDTYECEWKRAVTDPKVRARFRHFVNSEAGDDQVAFVPERGQIR
PASAQEKRAFRGIPVVVETE