Protein Info for ABZR86_RS03160 in Dyella japonica UNC79MFTsu3.2

Annotation: thiamine pyrophosphate-dependent enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 26 to 129 (104 residues), 86 bits, E=2.6e-28
• PF00205: TPP_enzyme_M
  • amino acids 223 to 313 (91 residues), 52.7 bits, E=5.9e-18
• PF02775: TPP_enzyme_C
  • amino acids 425 to 573 (149 residues), 95.5 bits, E=4.1e-31

Best Hits

KEGG orthology group: K04103, indolepyruvate decarboxylase [EC: 4.1.1.74] (inferred from 73% identity to cpi:Cpin_1754)

Predicted SEED Role

"Pyruvate decarboxylase (EC 4.1.1.1); Indole-3-pyruvate decarboxylase (EC 4.1.1.74)" (EC 4.1.1.1, EC 4.1.1.74)

MetaCyc Pathways

• superpathway of anaerobic sucrose degradation (17/19 steps found)
• pyruvate fermentation to acetate VIII (2/2 steps found)
• pyruvate fermentation to ethanol II (2/2 steps found)
• acetaldehyde biosynthesis II (1/1 steps found)
• L-methionine degradation III (2/3 steps found)
• indole-3-acetate biosynthesis VI (bacteria) (2/3 steps found)
• L-tryptophan degradation VIII (to tryptophol) (2/4 steps found)
• long chain fatty acid ester synthesis (engineered) (2/4 steps found)
• pyruvate fermentation to acetoin (1/3 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (5/9 steps found)
• butanol and isobutanol biosynthesis (engineered) (4/8 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.1 or 4.1.1.74

Sequence Analysis Tools

See A0A1I1ZK22 at UniProt or InterPro

Protein Sequence (590 amino acids)

>ABZR86_RS03160 thiamine pyrophosphate-dependent enzyme (Dyella japonica UNC79MFTsu3.2)
MADSNISNKSRSIATAPASISTATFTVADYLLTRLKQLNVTDVFQIPGDYVKHFTQALEY
FDGVNTIGAINELDAAYAADAYARTRGLAAVSLQFGVSTYSALNAIAGAWVERSPIVVIS
ATPGADMRDITDMYDVLFHHSTGDLNSDRAIYEYVTVKAITLSTNVGAAEQIDELLVDAI
TEKRPVYIACYKEVWGQPCERPPATPLKPRVTRSPELALRNAVDQAWAMITQAKSPLIFA
GVEVLRFGLSGLLQKIIDASGFLYTTTTLGKSVLDEQGDKFIGTYSDAASIEAVREIVMA
SDCVLTLGAIITDDYLTFIEAKYADMVLADTAGVRAGYFKYGDVTMRDFMEALLARFTAS
KAYPIKAKAPPQPKYPQPWAANSDPVYDAQPQIITYNRFFAHTMKFLQDKDLLKDIVMTY
GVSSSMYVATNCYGLKQGSFVSSAAWQCIGFETGAACGAQLGSGKRAWTVAGDGGFMMVC
QSLSTLARNQLNAVIFVMSNQVYAIEQVYVDMSAFKPGPKHKFDAFDILPKWDYLALAKA
FGAEGIRVETVDELNAALPRIAKIKDKPVLVEVVIPQKDLPGQMYRLGSE