Protein Info for ABIE53_005128 in Paraburkholderia graminis OAS925

Annotation: threonine aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 PF01212: Beta_elim_lyase" amino acids 15 to 295 (281 residues), 348.3 bits, see alignment E=5.2e-108 PF00155: Aminotran_1_2" amino acids 48 to 301 (254 residues), 29.8 bits, see alignment E=5.6e-11

Best Hits

Swiss-Prot: 56% identical to LTAE_ECO57: Low specificity L-threonine aldolase (ltaE) from Escherichia coli O157:H7

KEGG orthology group: K01620, threonine aldolase [EC: 4.1.2.5] (inferred from 96% identity to bug:BC1001_5230)

MetaCyc: 56% identical to low-specificity L-threonine aldolase (Escherichia coli K-12 substr. MG1655)
Threonine aldolase. [EC: 4.1.2.48, 4.1.2.5]; LTAA-RXN [EC: 4.1.2.48, 4.1.2.5, 4.1.2.49]; 4.1.2.48 [EC: 4.1.2.48, 4.1.2.5, 4.1.2.49]; Phenylserine aldolase. [EC: 4.1.2.48, 4.1.2.5, 4.1.2.49, 4.1.2.26]

Predicted SEED Role

"Low-specificity L-threonine aldolase (EC 4.1.2.48)" (EC 4.1.2.48)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.2.48, 4.1.2.5

Use Curated BLAST to search for 4.1.2.26 or 4.1.2.48 or 4.1.2.49 or 4.1.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (347 amino acids)

>ABIE53_005128 threonine aldolase (Paraburkholderia graminis OAS925)
MASHSKDALGHTMIDLRSDTVTRPTPAMLAAMSAAETGDDVWGDDPTVLRLQATLAERTG
KEAGLFFPSGTQSNLAALMAHCARGDEYIVGQLAHTYKYEGGGAAVLGSIQPQPLENAPD
GTIPLEKIAAAIKPIDNHFARTRLLALENTIGGKVLPESYVAQAVQLARQHGLSAHLDGA
RVFNAAVASGKPVAALCEPFDSVSICFSKGLGAPVGSVLVGSRALIDVAHRWRKVLGGGM
RQAGVLAAACLYALDHNVERLADDHANAAHLAAGLETIGQVKVQSVATNMVFAQLPQQHC
APLEAWLKERGILTQMLYASRFVTHKDVSRADIDTFIAAVKDYFAAR