Protein Info for ABIE53_004801 in Paraburkholderia graminis OAS925

Annotation: 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00170: 3-isopropylmalate dehydratase, large subunit
  • amino acids 1 to 468 (468 residues), 781.2 bits, E=1.5e-239
• PF00330: Aconitase
  • amino acids 7 to 460 (454 residues), 602.1 bits, E=4.2e-185

Best Hits

Swiss-Prot: 94% identical to LEUC_PARP8: 3-isopropylmalate dehydratase large subunit (leuC) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 98% identity to bpy:Bphyt_6850)

MetaCyc: 64% identical to isopropylmalate isomerase large subunit (Leptospira interrogans serovar Lai str. 56601)
(R)-2-methylmalate dehydratase. [EC: 4.2.1.35]; 3-isopropylmalate dehydratase. [EC: 4.2.1.35, 4.2.1.33]; 4.2.1.33 [EC: 4.2.1.35, 4.2.1.33]; RXN-7744 [EC: 4.2.1.35, 4.2.1.33]

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (469 amino acids)

>ABIE53_004801 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit (Paraburkholderia graminis OAS925)
MAQTLYDKLWNTHVIHTEEDGTTILYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAV
SDHNVPTTDRSHGIADPVSKLQVDTLDSNCDAYGITQFKMNDLRQGIVHIIGPEQGATLP
GMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQKKSKNMLVKVEGPLPRGCTA
KDIVLAIIGKIGTAGGTGYAIEFGGSTIRALSMEGRMTVCNMAIEAGARAGMVAVDDTTI
EYLKGRPFSPEGAEWDIAVDYWKQFKSDEGAQFDRVVELNAADIVPQVTWGTSPEMVTAV
DGRVPDPEREKDPGKREAMERALKYMALEPNAPIESIKPDKIFIGSCTNARIEDIRAAAY
VVKKLGRRVAPNIRLAMVVPGSGLVKAQAEREGLDKVFTDAGFEWREPGCSMCLAMNADR
LDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG