Protein Info for ABIE53_004646 in Paraburkholderia graminis OAS925

Annotation: squalene-hopene/tetraprenyl-beta-curcumene cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 680 transmembrane" amino acids 160 to 177 (18 residues), see Phobius details amino acids 198 to 214 (17 residues), see Phobius details TIGR01507: squalene-hopene cyclase" amino acids 38 to 669 (632 residues), 772.8 bits, see alignment E=2.6e-236 TIGR01787: squalene/oxidosqualene cyclases" amino acids 45 to 666 (622 residues), 618 bits, see alignment E=2.2e-189 PF13249: SQHop_cyclase_N" amino acids 46 to 336 (291 residues), 398.3 bits, see alignment E=4.8e-123 PF00432: Prenyltrans" amino acids 93 to 134 (42 residues), 29.3 bits, see alignment 1.4e-10 amino acids 440 to 458 (19 residues), 11.6 bits, see alignment (E = 5.1e-05) amino acids 510 to 548 (39 residues), 28.5 bits, see alignment 2.6e-10 amino acids 556 to 608 (53 residues), 29.9 bits, see alignment 9.7e-11 PF13243: SQHop_cyclase_C" amino acids 345 to 666 (322 residues), 493.8 bits, see alignment E=5e-152

Best Hits

Swiss-Prot: 64% identical to SQHC_BRADU: Squalene--hopene cyclase (shc) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K06045, squalene-hopene cyclase [EC: 5.4.99.17] (inferred from 92% identity to bgf:BC1003_4523)

MetaCyc: 66% identical to squalene hopene cyclase (Rhodopseudomonas palustris TIE-1)
Squalene--hopene cyclase. [EC: 5.4.99.17]; RXN-4961 [EC: 5.4.99.17, 4.2.1.129]

Predicted SEED Role

"Squalene--hopene cyclase (EC 5.4.99.17)" in subsystem Carotenoids (EC 5.4.99.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.129 or 5.4.99.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (680 amino acids)

>ABIE53_004646 squalene-hopene/tetraprenyl-beta-curcumene cyclase (Paraburkholderia graminis OAS925)
MNDLSQAHALGAAMPETAGEAPGAQATASTAAEASAALAPSLDAAITRATDAILAAQKPD
GHWVYELEADATIPAEYVLLVHYLGETPNVELEQKIARYLRRIQLPNGGWPLFTDGAIDI
SASVKAYFALKMIGDPVDAEHMVRAREAILAHGGAETVNVFTRILLALFGVVSWRAVPMM
PVEITLLPMWFPFHLSKVSYWARTVIVPLLVLNAKRPLARNPRRVRIDELFRGAPVNTGM
PARAPHQHVGWFGFFRVVDTVLRAVDGLFPKATRERAVREAVAFVDQRLNGEDGLGAIFP
AMANSVMMYDVLGYPADQPNRAIARRSIEKLLVIKDDEAYCQPCLSPVWDTSLAAHALLE
TGEARAEQAAERGLAWLRPLQILDVRGDWISRRPNVRPGGWAFQYNNAYYPDVDDTAVVA
MAMHRSEALTHSGADREAIARAREWVVGMQSSDGGWGAFEPENTQYYLNNIPFSDHGALL
DPPTADVSGRCLSMLAQLGEFPQNSEPAQRALDYMLKEQEADGSWYGRWGLNYIYGTWTA
LCSLNAAGLPHDDPRIRRAAQWLLSIQNEDGGWGEGGESYKLDYRGYERAPSTASQTAWA
LMGLMAAGEVDHEAVARGIEYLQREQREHGLWDETRFTATGFPRVFYLRYHGYRKFFPLW
ALARYRHLKRNGLTRVAVGM