Protein Info for ABIE53_004330 in Paraburkholderia graminis OAS925

Annotation: 2-oxoglutarate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 868 TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component" amino acids 28 to 864 (837 residues), 789.4 bits, see alignment E=2.2e-241 PF00676: E1_dh" amino acids 148 to 431 (284 residues), 120.4 bits, see alignment E=1.2e-38 PF02779: Transket_pyr" amino acids 512 to 716 (205 residues), 139.4 bits, see alignment E=1.5e-44 PF16870: OxoGdeHyase_C" amino acids 724 to 865 (142 residues), 120.7 bits, see alignment E=6.9e-39

Best Hits

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 88% identity to bgf:BC1003_5052)

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.2

Use Curated BLAST to search for 1.2.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (868 amino acids)

>ABIE53_004330 2-oxoglutarate dehydrogenase E1 component (Paraburkholderia graminis OAS925)
MSHVSSESIVLSRSPAGAVIEPAGDLSPVARTLQVRRFIDAFRQHGHRQASLDPLRLAPV
PEVPELDPVFHGLDPAQPIGAENPLLPPSAAVHELERQLRRVYCGAIGLDLTGIRDQQRR
AQLSAWLKTEQLAPLPAREPRRRLLRRLVAAETWERHVAARFEHAKRFSLEGCESLVPLI
EAIVDQAAAHGVRQVFAGMPHRGRLNVLANVMDFDPAKLVDCLDPDSEAAAVQRDLPYHL
GASTVRKTQDGDVAIFLAPNPSHLQSVYPVVSGMARAYQDEHRGFGCLPLVMHGDAAFAG
QGIVMETLNMTRKDGYALGGTIHVIVNNQIGFTTPNRMDAEAYSYCTDIARMIDAPVIRV
NADRPDEVMRAAKLALQYRMEHEADVIIDLIGYRRLGHSEHDIPALTQPLQQAAIAMHQT
VTELYHAAIGEETPLDVLRAEALKELQTTPPSARTSSRSHGNRIKRTRPLSLARMQSLTA
ALTRTPPAFRLHELLRNITAQWQKSVADETAAVDWCFAENLAYATLLDDGHSVRISGMDV
GRGTFMHRHAVWHSQGNAAGNAASDTANATEPYVPLQNLGVHQGEFDIVNSPLTEEAVLG
FEYGYSVQTGSHLAIWEAQFGDFVNGAQVIIDQYIAAGEYKWDQRSGLTMLLPHGHEGVG
PEHSTGYVSRFLQLCAGGNMKVVMPSTSSQWFHMLREQALSAEPKPLVVMSPKGQLYGNA
RSHSPLGELIGGAFLPVLEDSGVASPQDVTRVVLSSGKFFYDLQAARDAGGDTRTALIRV
EQLYPFPTSELAVALAAFPQLEEVVWAQEEEKKHGAWHFVRDEIEQAVPANCRIVDVCRR
ESPSGAHSSIRAHRDEQQRLVAAALARG