Protein Info for ABIE53_004291 in Paraburkholderia graminis OAS925
Annotation: aspartate-semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to DOEC_HALED: Aspartate-semialdehyde dehydrogenase (Non-phosphorylating) (doeC) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)
KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 95% identity to bgf:BC1003_5088)MetaCyc: 54% identical to aspartate-semialdehyde dehydrogenase (non-phosphorylating) (Halomonas elongata DSM 2581)
1.2.1.M5 [EC: 1.2.1.M5]
Predicted SEED Role
"Aldehyde dehydrogenase B (EC 1.2.1.22)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism (EC 1.2.1.22)
MetaCyc Pathways
- 4-aminobutanoate degradation III (2/2 steps found)
- methylglyoxal degradation V (2/3 steps found)
- L-lactaldehyde degradation (aerobic) (1/2 steps found)
- lactate biosynthesis (archaea) (3/5 steps found)
- L-rhamnose degradation II (5/8 steps found)
- ectoine degradation (2/4 steps found)
- methylglyoxal degradation IV (1/3 steps found)
- superpathway of methylglyoxal degradation (4/8 steps found)
- L-canavanine degradation II (1/6 steps found)
- superpathway of fucose and rhamnose degradation (5/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.16, 1.2.1.22
Use Curated BLAST to search for 1.2.1.16 or 1.2.1.22 or 1.2.1.M5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (500 amino acids)
>ABIE53_004291 aspartate-semialdehyde dehydrogenase (Paraburkholderia graminis OAS925) MLLGHPVLFKSLCYVDGRWVHSASAASVAVRNPADQEVLGHVPMLDAAQITEAVDAAQRA FSAWRWLPVAKRSALLLRWHELILRHQHDLAAILSLEQGKPFAEARGEIAYGASFVEWFA HEVRRLNGRTIPTHIDGAQLGTVIEPVGVAALITPWNFPCAMITRKAAAALAAGCTVVVK PAHETPFSALALAQLAEEAGFPAGVFNVVLGEPQMAMQTLVGDSRVRSVSFTGSTRVGGL VMQAVANAGIKKTALELGGNAPFIVTEDADLEQAVRVAIAAKFQTSGQDCCAANRIFVAR PLYETFVTRYSDAVRALKVGSAFEADVDVGPLMHQAAFDATRQRVADAQAKGARITVGGS AHERGGWFFEPTVVADAAPGMRIYDEENFAPISALTPFDSLDEVIERANDTEYGLAAYVC ARDLNTVFQLIRRLDFAMVSVNGAKFTGAPIPFGGIKSSGLGREGGAEGFEPFVETRYFC LGQLGLPVTQSLPARSAAAA