Protein Info for ABIE53_004046 in Paraburkholderia graminis OAS925

Annotation: pyruvate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 611 PF02776: TPP_enzyme_N" amino acids 4 to 133 (130 residues), 110.8 bits, see alignment E=7.1e-36 PF01855: POR_N" amino acids 46 to 127 (82 residues), 28 bits, see alignment E=3.7e-10 PF00205: TPP_enzyme_M" amino acids 211 to 341 (131 residues), 92.4 bits, see alignment E=4.3e-30 PF02775: TPP_enzyme_C" amino acids 402 to 557 (156 residues), 99.3 bits, see alignment E=3.7e-32

Best Hits

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 97% identity to bug:BC1001_4081)

Predicted SEED Role

"Pyruvate oxidase (EC 1.2.3.3)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.5.1

Use Curated BLAST to search for 1.2.3.3 or 1.2.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (611 amino acids)

>ABIE53_004046 pyruvate dehydrogenase (quinone) (Paraburkholderia graminis OAS925)
MSTTVGDFIVERLQAWGVRRIFGYPGDGINGVFGALNRAQSEAKKHHKNTDQPGPIEFIQ
VRHEEMAAFMASAHAKFTGELGVCIATSGPGASHLVTGLYDARMDHMPVLAITGQQARAA
LGGHYQQEVDLISLFKDVAGAFVGQASVPAQVRHLVDRAIRTALSERKVTALVLPNDLQD
LPYEPPARKHGTLHSGVGYREPRLVPQPEDLQQAADVLNAGEKVAILVGAGALHATDEVI
AVAEKLGAGVAKALLGKAVLPDDLPWVTGSIGLLGTKPSYDLMTECDTLLMIGSGFPYSE
FLPKEGAARGVQIDLKADMLSLRYPMEVNLVGDSVETLRALLPLLEHKQDRGWRKTIEGW
TADWWKTLDERAHEPGKDAVNPQRTVWELSPRVPSNAIVTSDSGSCANWYARDLKVKRGM
MCSLSGGLASMGAAVPYAIAAKFAYPERPVIALVGDGAMQMNNMAELITVSKYWKGWADP
RWICMVLNNQDLNQVTWEQRVMEGDPKFEASQDIPSVPYHKFAELIGLKGIYVDDAEQMG
AAWDAALAADRPVVIEVKADPNIAPLPPHITLAQAKAFASTLLKGDPDEGNVIVQTAKQV
LGAVLPGRHDK