Protein Info for ABIE53_003618 in Paraburkholderia graminis OAS925

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 29 to 503 (475 residues), 482.3 bits, see alignment E=8.8e-149 PF08245: Mur_ligase_M" amino acids 115 to 324 (210 residues), 187.4 bits, see alignment E=3.1e-59 PF02875: Mur_ligase_C" amino acids 345 to 437 (93 residues), 86 bits, see alignment E=1.8e-28

Best Hits

Swiss-Prot: 59% identical to MURE_RALSO: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 93% identity to bug:BC1001_3114)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (515 amino acids)

>ABIE53_003618 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase (Paraburkholderia graminis OAS925)
MSALRNNHPSHRQIAEALNWLQAHLQRGAHLHADTRSLTAGDAFFAYAVDGADNRAFIDG
ALERGAAAVLVQPEGFSGAIDASTTLAVPALNELAGSIASAWYGDPSDSMLTVGVTGTNG
KTSCSQWISAALTALGKRCAIIGTLGTGLPGQLVHTGFTTPDAPQLQRSLAQLRDAGAKA
VAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHHTFEAYEAAKARLFAWPELRAAVI
NRDDAAGRRLLASTQGHARTIAYGLDSAQQSAPQADASLLASNVRATPTGTAFHLSTSDW
GNAEVEVQTLGAFNVSNLLGVLGALLAAEIPFDAALAELAKLESVNGRMQRLGGRLQNDE
PLVVIDYAHTPDALEKTLEALRPMAAARGGELICMFGCGGDRDATKRPLMGAIAEKMADG
VVVTSDNPRSEDPQSIIEQIAAGMKDASKARRIEDRASAILQAIRGAAREDVIVLAGKGH
EATQEIMGKKRAFSDQDHARLALAARATQARGGGE