Protein Info for ABIE53_003617 in Paraburkholderia graminis OAS925

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 33 to 76 (44 residues), 23 bits, 1.3e-08
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 36 to 459 (424 residues), 404.2 bits, E=3.4e-125
• PF08245: Mur_ligase_M
  • amino acids 113 to 302 (190 residues), 137.1 bits, E=1.2e-43
• PF02875: Mur_ligase_C
  • amino acids 323 to 409 (87 residues), 50.2 bits, E=3.9e-17

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 92% identity to bug:BC1001_3113)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Protein Sequence (475 amino acids)

>ABIE53_003617 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (Paraburkholderia graminis OAS925)
MSAQGNAMFTLREAAALIPGATVIGDDSVAFERVSTDSRSAGPGDLFVAIKGDRFDAHDF
LADVAARNVSAALVARVPGGWSVPALRVADTRVGLGALARGWRRKFSMPLVAVTGSNGKT
TVKEMIASIFAAAVGVDARLATAGNFNNDIGLPLTLFRLNAAHRLAVVELGMNHPGETSL
LAKIAEPTVAVVNNAQREHQEFMATVEAVALEHASVIHALTPDGIAVFPADDAYASIWRV
AATGNRIVDFALNTAERTTEAAVTGTFDGKVLSIQTPEGHTNITLQVLGNHNAHNALAAT
SAALAAGVSLDAIKRGLESFGAVKGRLQVKHAALGTLAGATVIDDTYNANPDSMRAAIDV
LASHASPRVLVMGDMGEVGDNGPEFHREIGAYAKERGIDALYAMGDASRDACTAYGASAH
HVADVGTLVAQLQQAGFGPAATFLVKGSRFMQMERVVDAVTSPQPNAAGSAPAAH