Protein Info for ABIE53_003612 in Paraburkholderia graminis OAS925

Annotation: UDP-N-acetylmuramate--alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 7 to 457 (451 residues), 553.2 bits, E=2.7e-170
• PF01225: Mur_ligase
  • amino acids 7 to 105 (99 residues), 111.5 bits, E=3.1e-36
• PF08245: Mur_ligase_M
  • amino acids 110 to 290 (181 residues), 91.7 bits, E=9.4e-30
• PF02875: Mur_ligase_C
  • amino acids 311 to 403 (93 residues), 52.4 bits, E=8.3e-18

Best Hits

Swiss-Prot: 97% identical to MURC_PARXL: UDP-N-acetylmuramate--L-alanine ligase (murC) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 98% identity to bgf:BC1003_3055)

MetaCyc: 54% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Protein Sequence (467 amino acids)

>ABIE53_003612 UDP-N-acetylmuramate--alanine ligase (Paraburkholderia graminis OAS925)
MKHIVKHIHFVGIGGVGMSGIAEVLLNLGYQVSGSDLSSNAVTDRLAALGARIAIGHAAE
NIEGANAVVVSTAVRSDNPEVLAARHRRIPIVPRAVMLAELMRLKQGIAIAGTHGKTTTT
SLVASVLAAGGLDPTFVIGGRLISAGANARLGTGDFIVAEADESDASFLNLFPVIEVITN
IDADHMDTYGHDFARLKQAFIEFTHRLPFYGIAVLCVDDPNVKEILPFVSKPIIRYGLAP
DAQVRAVNVEARDGKMHFTAMREDAAPLDIVLNLPGEHNVQNALAAIAIATELEVKDADI
QRALADFNGVGRRFQRYGEVPVTSDGEPRGAYTLVDDYGHHPVEMAATVAAARGAFPGRR
LVLAFQPHRFTRTRDCFEDFVKVLSTVDALVLTEVYSAGEAPIVAADGRALARALRVAGK
VEPVFVDTVDEVPDALAALVRDGDVVITMGAGSIGGVPGRLAQETKV